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Animal nucleic acid sample normal temperature preservation reagent and application thereof

A nucleic acid sample storage technology at room temperature, applied in the field of animal nucleic acid sample storage reagents at room temperature, can solve the problems of cold chain transportation, biological safety hazards, low efficiency, etc., and achieve the effect of ensuring personal safety, protection safety, and simple process

Inactive Publication Date: 2014-04-30
SHANGHAI JIAO TONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The on-site collection of these disease materials requires specialized quarantine personnel to operate. The cost is high, the efficiency is low, and the transportation requires a cold chain, etc., and there are many biological safety hazards such as the spread of pathogens along with transportation.

Method used

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  • Animal nucleic acid sample normal temperature preservation reagent and application thereof
  • Animal nucleic acid sample normal temperature preservation reagent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Preparation of reagent 1 for animal nucleic acid samples stored at room temperature: In 293ml of water, add 250g of guanidine isothiocyanate, 17.6ml of sodium citrate with a concentration of 0.75mol / L, and 26.7ml of sodium dodecyl sulfate with a mass fraction of 10%. aqueous solution and 50ml of 2mol / L sodium acetate solution, the resulting mixture was mixed with 500ml of phenol, stirred evenly, and titrated with 0.1mmol / L hydrochloric acid until the pH value was 7.0.

Embodiment 2

[0041] Preparation of reagent 2 for animal nucleic acid samples stored at room temperature: In 293ml of water, add 250g of guanidine isothiocyanate, 17.6ml of 0.75mol / L sodium citrate, 14.5g of 0.1% 8-hydroxyquinoline, 3.9g of 0.5 % of β-mercaptoethanol, 36.7ml of 10% aqueous solution of sodium lauryl sulfate, and 50ml of 2mol / L sodium acetate solution, mix the resulting mixture with 500ml of phenol, stir well, and use a concentration of 0.1mmol / L of sodium hydroxide solution Titrate to pH 9.5.

Embodiment 3

[0043] Handling of the filter paper used in the sample collection / storage area of ​​the animal nucleic acid sample collection / storage card:

[0044] Step 1): Put 903 # Soak the filter paper in the animal nucleic acid sample prepared in Example 1 for 1 minute at room temperature;

[0045] Step 2): Bake the filter paper soaked in step 1) at 37-50°C for 1-3 minutes; sterilize;

[0046] Then place the treated filter paper in the sample collection / preservation area of ​​the animal nucleic acid sample collection / preservation card for use.

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Abstract

The invention discloses an animal nucleic acid sample normal temperature preservation reagent and an application thereof. The animal nucleic acid sample normal temperature preservation reagent comprises an acid cell lysis agent, nuclease inhibitors, a surfactant, sodium citrate and sodium acetate, wherein the acid cell lysis agent is guanidinium isothiocyanate, the used nuclease inhibitors are 8-hydroxyquinoline and beta-mercaptoethanol, and the used surfactant is lauryl sodium sulfate. On the other hand, the invention discloses a method for treating filter paper by using the animal nucleic acid sample normal temperature preservation reagent. The method comprises the following steps: 1) soaking the filter paper in the animal nucleic acid sample normal temperature preservation reagent; 2) baking the filter paper at 37-50 DEG C; 3) sterilizing the filter paper for later use.

Description

technical field [0001] The invention relates to a reagent for preserving nucleic acid samples, in particular to a reagent for preserving animal nucleic acid samples at room temperature and an application thereof. Background technique [0002] Nucleic acid is a biological macromolecular compound synthesized by many nucleotides, and is one of the most basic substances of life. Nucleic acid macromolecules can be divided into two categories: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), which store and transmit genetic information in protein replication and synthesis. Nucleic acid is not only the basic genetic material, but also plays an important role in the biosynthesis of proteins, so it plays a decisive role in a series of major life phenomena such as growth, heredity, and variation. As the central dogma of molecular genetics reveals the relationship between nucleic acid and protein, the range of applications for nucleic acid analysis and research experiments such...

Claims

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Application Information

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IPC IPC(8): C12N15/10C12M1/00
Inventor 崔立刘雨潇赖志马晶晶王春艳高俊锋
Owner SHANGHAI JIAO TONG UNIV
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