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Milk beverage containing bifidobacteria embedded beads and preparation method thereof

A technology of bifidobacteria and bifidobacterium longum, applied in dairy products, milk preparations, applications, etc., can solve the problems of low activity of bifidobacteria, short shelf life, difficult storage, etc., achieve the effect of probiotics, and the method is simple , The effect of stable product quality

Active Publication Date: 2014-05-07
GANSU ACAD OF SCI INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a milk drink with bifidobacterium-embedded beads to solve the problems of low activity of bifidobacteria, short shelf life and difficult storage in bifidobacteria milk beverage products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Preparation of bifidobacterium-embedded beads

[0042] A. Bacteria activation test tube culture

[0043] The medium consists of 15g peptone, 10g beef extract, 3g tryptone, 1g yeast extract powder, 15g glucose, 5g dipotassium hydrogen phosphate, 0.5g sodium acetate, 0.5g diammonium hydrogen citrate, 0.1 g zinc sulfate heptahydrate, Magnesium sulfate heptahydrate 0.05g, L-cysteine ​​hydrochloride 0.1g, fructooligosaccharide 0.5g, calcium carbonate 0.5g, Tween-80 5g and water 1000g. The pH value of the medium is 7.0, and it is used after being sterilized by moist heat at 0.05MpaMpa for 30 minutes;

[0044] Before the strain activation, Bifidobacterium longum subsp. longum ( Bifidobacterium longum subsp. longum ) ampoule bottle at room temperature for 2 hours, then wipe the surface of the ampoule bottle with a 75% alcohol cotton ball in a sterile room for disinfection, open the ampoule bottle, draw 0.2 mL of medium into the ampoule bottle with a sterile pipette, and p...

Embodiment 2

[0064] (1) Preparation of bifidobacteria embedding block

[0065] A. Bacteria activation test tube culture

[0066] The medium consists of 15g of peptone, 10g of beef extract, 3g of tryptone, 1g of yeast extract powder, 15g of glucose, 5g of dipotassium hydrogen phosphate, 0.5g of sodium acetate, 0.5g of diammonium hydrogen citrate, 0.1g of zinc sulfate heptahydrate, Magnesium sulfate heptahydrate 0.05g, L-cysteine ​​hydrochloride 0.1g, fructooligosaccharides 0.5g, calcium carbonate 0.5g, Tween-80 5g and water 1000g; the pH value of the medium is 7.5, at 0.14Mpa Use after 15 minutes of moist heat sterilization;

[0067] Before the strain activation, Bifidobacterium longum subsp. infantis ( Bifidobacterium longum subsp. infantis ) ampoule bottle at room temperature for 3 hours, then wipe the surface of the ampoule bottle with a 75% alcohol cotton ball in a sterile room for disinfection, open the ampoule bottle, draw 0.25mL of medium into the ampoule bottle with a sterile pip...

Embodiment 3

[0088] (1) Preparation of bifidobacterium-embedded beads

[0089] A. Bacteria activation test tube culture

[0090] The medium consists of 15g peptone, 10g beef extract, 3g tryptone, 1g yeast extract powder, 15g glucose, 5g dipotassium hydrogen phosphate, 0.5g sodium acetate, 0.5g diammonium hydrogen citrate, 0.1 g zinc sulfate heptahydrate, Magnesium sulfate heptahydrate 0.05g, L-cysteine ​​hydrochloride 0.1g, fructooligosaccharide 0.5g, calcium carbonate 0.5g, Tween-80 5g and water 1000g. The pH value of the medium is 6.0, and it is used after being sterilized by moist heat at 0.10Mpa for 20 minutes;

[0091] Before the strain activation, Bifidobacterium adolescent ( Bifidobacterium adolescentis ) ampoule bottle at room temperature for 2.5 h, then wipe the surface of the ampoule bottle with a 75% alcohol cotton ball in a sterile room for disinfection, open the ampoule bottle, draw 0.3 mL of medium into the ampoule bottle with a sterile pipette, and put The bacteria in th...

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Abstract

The invention discloses a milk beverage containing bifidobacteria embedded beads and a preparation method of the milk beverage to solve the problems of low activity, short storage time and difficulty in storage of bifidobacteria in a bifidobacteria milk beverage product. The milk beverage contains the bifidobacteria embedded beads. The preparation method comprises the following two steps: firstly, preparing the bifidobacteria embedded beads; secondly preparing the milk beverage containing the bifidobacteria embedded beads. According to the milk beverage, the problems of low viable count, short storage time, difficulty in reaching the intestinal tract and the like of the bifidobacteria in the milk beverage are effectively solved, the viable count, the activity and the storage time of the bifidobacteria are effectively ensured, and the probiotic effect of the bifidobacteria on a human body is achieved. The preparation method is mild in preparation conditions, is simple and convenient, and is suitable for large-scale industrialization production.

Description

technical field [0001] The invention relates to a milk drink containing bifidobacterium-embedded beads and a preparation method thereof. Background technique [0002] Bifidobacterium is a normal physiological microorganism in the human body. It colonizes in the intestinal tract and is the dominant flora in the intestinal tract. The number of bifidobacteria is closely related to human health. Sexual beneficial bacteria. The main physiological functions of bifidobacteria are: 1. Biological barrier and biological antagonism; 2. Enhance immunity and anti-tumor effect; 3. Nutritional effect; 4. Inhibit the production of endotoxin; 5. Slow down the aging of the body. With the increase of age, environmental pollution and the use of antibiotics, the number of bifidobacteria in the human intestinal tract gradually decreases, resulting in the destruction of the micro-ecological balance in the intestinal tract, leading to the occurrence of various diseases and aging. [0003] The mos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23C9/13A23C9/152
Inventor 邵建宁麻和平刘彩云张文齐赵昊星魏亚琴彭章普
Owner GANSU ACAD OF SCI INST OF BIOLOGY
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