Device and method applied in gradient temperature control

Abstract

The invention provides a device applied in gradient temperature control. The device is composed of a first temperature control module, a second temperature control module, a control module, a transmission device or a rotation device, a controlled target and a detection module. The method and device of the invention has the advantage of good expandability, different temperature control modules can be alternately used at different time points, the temperature control module in the off-working state adopts the idle time to realize the next target temperature value, sudden changes do not needed, and the service life can be prolonged; the temperature control module in the working state does not undergo the temperature rising / falling process, so the sample module temperature rising / falling rate can be improved, the overshoot phenomenon can be avoided, and the complex control algorithm does not needed. The temperature control is more flexible, the effective temperature control time of the controlled target can be shortened, the temperature change rate of the controlled target can be improved, and the application in gradient temperature control can be realized. Less temperature control modules are adopted, and different temperature control modules are used to control the temperature of the controlled target in different time periods through the idea of 'time division multiplex access' so as to realize a plurality of target gradient temperatures.

Description

technical field [0001] The invention belongs to the field of temperature control, and relates to a gradient temperature control device and method, which can be applied to occasions where a controlled target needs to realize multiple target temperature values. For example, the polymerase chain reaction (PCR) in the fields of biology and medicine requires the target sample to achieve three processes of high temperature denaturation, low temperature annealing, and medium temperature extension, corresponding to three different gradient temperatures, and these three different gradient temperatures are required to achieve Multiple thermocycling to complete the PCR amplification reaction. Background technique [0002] Some related technologies in many fields such as biology, chemistry, medicine, industry, etc. need to use thermal cycle to realize. [0003] Take the "polymerase chain reaction" (PCR), which is common in the field of biology, as an example. In order to realize the P...

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Problems solved by technology

The disadvantages of this method are: (1) use the same temperature control module to achieve heating and cooling in a short period of time, and sudden changes in temperature can easily damage the temperature control module and shorten its service life; (2) During the temperature control process of the sample module (that is, the controlled target, the same below), the temperature control module itself has a process of heating and cooling, which affects the rate of heating and cooling of the sample module. Temperature has overshoot phenomenon, which requires complex control algorithm to eliminate or weaken this phenomenon

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