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Structured light lighting optical system

A technology of structured light illumination and optical system, applied in the field of optical imaging, can solve the problems of high cost of spatial light modulator, limited imaging speed, large system volume, etc., achieve high-speed switching and phase shift capability, reduce system volume and cost, The effect of avoiding the effects of system stability

Active Publication Date: 2014-05-14
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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Problems solved by technology

However, spatial light modulators have disadvantages such as high cost, limited imaging speed, and large system volume.

Method used

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Embodiment Construction

[0045] The present invention will be specifically introduced below in conjunction with the accompanying drawings and specific embodiments.

[0046] figure 2 It is a structural schematic diagram of an embodiment of the present invention.

[0047] Such as figure 2Shown: a structured light illumination optical system, including: LED excitation light source 3 with multiple chip structures; connected to LED excitation light source 3 and independently controlling the brightness and darkness of each chip, and then controlling the entire LED excitation light source 3 to form brightness and darkness The computer 1 of streak light; the relay lens group 5 positioned on the emission light path of bright and dark streak light; comprising a plurality of fly-eye lens units 41, and each fly-eye lens unit 41 receives the bright and dark streaks passing through the relay lens group 5 The light is used to image the multiple chip structures of the LED excitation light source 3, thereby formin...

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Abstract

The invention discloses a structured light lighting optical system. The structured light lighting optical system comprises a computer, a synchronous control system, an ultra-high brightness LED excitation light source, a relay lens set, a fly-eye lens array, a middle image plane, a dichroscope, a projection objective, an objective table, a tube mirror, a spike filter and a photoelectric detector. A multi-chip structure of the ultra-high brightness LED excitation light source is controlled by the adoption of the computer to obtain initial strip light, the number of strips is multiplied through the relay lens set and the fly-eye lens array, and the light is imaged on the lighting objective table through the projection objective according to a certain magnification to form structured light lighting. Structured light lighting is achieved by adopting the mode of electrical control over LED chips, the disadvantages, of being high in cost, large in size and low in strip phase shifting speed, caused by the adoption of an optical grating and a spatial light modulator are abandoned, and the structured light lighting optical system can be applied to a rapid real-time three-dimensional imaging research on living body biological cells.

Description

technical field [0001] The invention relates to a novel structured light illumination optical system in super-resolution microscopic imaging technology, specifically a structured light illumination microscope imaging optical system based on a fly-eye lens array, which belongs to the field of optical imaging technology. Background technique [0002] Ordinary optical microscopes are limited by the optical diffraction limit. The lateral resolution can only reach 200nm, and the vertical resolution is about 500nm. It is difficult to meet the research needs of subcellular and molecular structures in the current biological field. [0003] Structured light imaging technology was first proposed by Neil et al. in 1997, and applied to biological imaging, the resolution can reach 100nm. This technology uses a specially modulated structured light field to illuminate the sample, and extracts the information of the focal plane from the modulated image data of different phases by using the ...

Claims

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Application Information

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IPC IPC(8): G02B21/06
Inventor 熊大曦杨西斌
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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