High-throughput detection method for grain form parameters

A detection method and high-throughput technology, applied in the field of crops, can solve problems such as insufficient grain shape parameters, and achieve the effects of accurate and reliable detection results, improved detection efficiency and stability, and efficient and accurate detection.

Active Publication Date: 2014-05-21
BEIJING PAIDE WEIYE TECH DEV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

And the particle shape parameters analyzed are not comprehensive enough

Method used

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  • High-throughput detection method for grain form parameters
  • High-throughput detection method for grain form parameters

Examples

Experimental program
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Effect test

Embodiment 1

[0061] A high-throughput detection method for grain shape parameters, comprising the following steps,

[0062] Step 1. Build a particle shape detection platform, such as figure 1 as shown,

[0063] On the horizontal workbench, fix the digital single-lens reflex camera 2 with the help of a remake stand 1 or a tripod;

[0064] The imaging plane of the above-mentioned digital single-lens reflex camera 2 is parallel to the workbench, and the height of the camera is set; the set height is used as a standard to obtain a more ideal imaging.

[0065] Place a tray 7 with a monochromatic background on the plane of the horizontal workbench to carry the grains to be tested; the color of the tray 7 is advisable to have a larger contrast with the color of the grains;

[0066] The digital SLR camera 2 is connected with the computer 4 through the camera connection line 3;

[0067] Turn on the real-time view mode of the digital SLR camera to display the real-time imaging information of the ca...

Embodiment 2

[0101] 1. Build the shooting environment according to the specifications provided by the technical solution of the present invention, start the image processing software in the computer (hereinafter referred to as the software) and establish a connection with the camera, and switch the software to the "live view" mode.

[0102] 2. Place the calibration reference object in the grain tray, control the camera to capture the image of the reference object; the image of the reference object is sent back to the computer, and the software switches to the "analysis" mode.

[0103] 3. Select the calibration function, left-click the starting point of the scale in the reference object image; move the mouse to the end point of the scale, and click the left mouse button again; at this time, the pixel connection line between the start point and the end point is drawn in the reference object image and displayed The number of pixels between two points. If you are not satisfied with this select...

Embodiment 3

[0112] 1. Use the calibrated grain shape parameters to detect the environment, spread the sample grains in the grain tray, observe the samples to be tested through the "real-time view" function, and when the sample distribution meets the detection requirements, control the camera to collect grain images and send them back To the computer, switch the detection software to "analysis" mode.

[0113] 2. Select the "parameter detection" function, and the software will load the conversion rate and segmentation threshold saved by the software to perform image segmentation; the segmented grain target area will be labeled and its outline will be obtained, and then the morphological characteristic parameters of the grain will be obtained; and according to the setting Set the threshold for noise screening.

[0114] 3. The parameter detection result will be superimposed with the collected grain image and displayed in the main work area, and the area label will be displayed in the lower ri...

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Abstract

The invention discloses a high-throughput detection method for grain form parameters. The high-throughput detection method includes the steps: setting up a grain form detection platform; calibrating a detection environment; acquiring and segmenting an image; extracting a contour and acquiring characteristic parameters; sieving adhered grains and impurities by the aid of a grain form parameter preset threshold; analyzing an image in the contour and realizing high-throughput color and texture characteristic detection; outputting grain form parameter detection results. The detection results are accurate and reliable, and compared with a manual measuring method, the grain form parameter detection method greatly improves detection efficiency and stability. The detection requirement for the grain form parameters in gene research can be met, the method is simple to implement, detection is efficient and accurate, and application requirements are met.

Description

technical field [0001] The invention relates to the field of crops, in particular to a high-throughput detection method for grain shape parameters. Background technique [0002] Currently, the shape and size of cereal grains is one of the most important agricultural traits, not only affecting yield and eating quality, but also an important determinant of market price. Therefore, botanical research fields such as genetics, gene function analysis, molecular breeding and crop improvement all need to quantitatively evaluate the morphological characteristics of seeds. Efficient, reliable, high-throughput methods for measuring grain shape phenotypes are required. [0003] In general, the morphological characteristics of cereal grains are mainly measured by two methods. The simplest method is the method introduced in the national standard GB / T 17891-1999, "take 10 grains of polished rice that are intact and undamaged as random numbers, place them flat on the measuring board, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01B11/00G01B11/24
Inventor 吴建伟杨宝祝明博申光磊辛颖韩建冰彭浩孙明军
Owner BEIJING PAIDE WEIYE TECH DEV
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