Nanometer mimic enzyme immunochromatography detection method

An immunochromatographic detection and enzyme-simulating technology, applied in the fields of nanomaterials and biomedical nanometers, can solve the problems of high price and restricting the development and promotion of magnetic immunochromatography

An immunochromatographic detection and enzyme-simulating technology, applied in the fields of nanomaterials and biomedical nanometers, can solve the problems of high price and restricting the development and promotion of magnetic immunochromatography

CN103808926AActive Publication Date: 2014-05-21重庆康巨全弘生物科技有限公司
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  • Nanometer mimic enzyme immunochromatography detection method
  • Nanometer mimic enzyme immunochromatography detection method
  • Nanometer mimic enzyme immunochromatography detection method

Examples

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Embodiment

[0052] Example: Detection of abrin toxin by nano-mimetic enzyme immunochromatography detection method

[0053] 1) Preparation of hybridoma cells

[0054] Acacia toxin is an ingredient in the seeds of the leguminous plant Acacia acacia. It is one of the most toxic plant toxins found so far. It is very toxic to humans, animals and insects. Chewing a piece of Acacia acacia seed is enough As a result, the present invention uses the detection of abrin toxin (abbrin toxin and ricin toxin mentioned below are provided by the Academy of Military Medical Sciences) as an example to illustrate the sensitivity and practicability of the nano-mimetic enzyme immunochromatographic detection method. The monoclonal antibodies Abrin-1, Abrin-2, Abrin-3, and Abrin-4 against abrin-toxin used in the experiment were derived from the secretory monoclonal antibodies Abrin-1, Abrin-2, Abrin-3, and Abrin-4, respectively. Hybridoma cells (methods for the production of monoclonal antibodies are known in t...

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Abstract

The invention provides a nanometer mimic enzyme immunochromatography detection method for detecting a to-be-detected object in a liquid sample. The method sequentially comprises the following steps: (1) providing a detecting probe which is prepared by coupling magnetic nanometer particles with a first molecule capable of being specifically bond with a to-be-detected object; (2) providing a capturing probe which is a fixed second molecule capable of being specifically bond with the to-be-detected object; (3) allowing the liquid sample to have contact with the detecting probe; (4) allowing the liquid sample which has contact with the detecting probe to have contact with the capturing probe; and (5) adding a hydrogen supply substrate and peroxide into the capturing probe which is subjected to the treatment in step (4) and performing a chromogenic reaction. The invention also provides a nanometer mimic enzyme immunochromatography detecting device for detecting the to-be-detected object in the liquid sample.

Description

technical field [0001] The invention belongs to the field of nanometer material and biomedical nanotechnology. The invention relates to a magnetic nanoparticle imitation enzyme, and provides a method for its application in immunochromatographic biomolecule detection. Background technique [0002] Colloidal gold immunochromatography is a detection method developed in the early 1990s with colloidal gold as a marker. It combines immunoaffinity technology, printing technology and dot thin layer chromatography technology. When using this chromatographic strip for detection, all samples go through the continuous reaction of the narrow cellulose membrane, which actually concentrates and aggregates the tested substances, improves the sensitivity of the reaction, and speeds up the reaction speed. The operation time is only 3 to 15 minutes. The principle is to fix the specific antibody (or antigen) on a certain zone of the nitrocellulose membrane first, and when one end of the dry n...

Claims

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Application Information

Patent Timeline
21 May 2014
Publication
CN103808926A
IPC
G01N33/558
CPC
G01N33/54326; G01N33/54346; G01N33/54388; G01N33/5308; G01N33/56983; G01N33/577; G01N2333/11; G01N2333/42
Inventors
阎锡蕴; 段德民