A method for quantitative detection and tracking of intracellular hap nanoparticles

Abstract

The invention relates to a rare earth fluorescent signal based quantitative detection method of HPA (hydroxyapatite) nano particles, and the quantitative detection method is characterized by comprising the following steps: 1) formulating a europium ion fluorescence-concentration standard curve; 2) establishing dissolution rate of calcium ions and europium ions in the HPA (hydroxyapatite) nano particles, and determining the accuracy of a fluorescence-concentration standard curve method; 3) quantitatively detecting intracellular HAP nano particles; and 4) tracing a dissolution course of the intracellular HAP nano particles. The main advantages of the quantitative detection method are that: 1) the quantitative detection method is good in biocompatibility, safe and reliable, and is free of radioactive labeling using condition limitation problems; 2) the quantitative detection method is low in the detection limit, the detection limit concentration can reach 0.5nM, and the sample needing amount is less; and 3) the quantitative detection method can more accurately shown dissolution of the intracellular HAP nano particles, and the compatibility of the dissolving out of the europium ions and the dissolution of the HAP nano particles is high.

Description

technical field [0001] The invention relates to a quantitative detection method of hydroxyapatite nanoparticles based on rare earth fluorescence signals, which can be used for the quantitative detection of intracellular apatite nanoparticles and the tracing of the dissolution process. Background technique [0002] Hydroxyapatite (HAP) has good biocompatibility and biological activity, especially HAP nanoparticles have strong adsorption capacity for drugs, proteins or genes due to their high specific surface area, and HAP nanoparticles have strong adsorption capacity in cells The acidic environment of lysosomes (pH value around 5.0) also exhibits higher solubility than bulk HAP, so HAP nanoparticles can be regarded as a biocompatible, intracellularly degradable inorganic carrier material. For HAP nanoparticles, in addition to the related preparation technology research, the amount of HAP nanoparticles entering the cell, the dissolution process of HAP nanoparticles in the cell...

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a method for the quantitative detection and tracing of intracellular HAP nanoparticles in view of the above-mentioned prior art, to avoid the defects of radioisotope labeling that have potential safety hazards and limited use conditions, and to solve the problem of polymer fluorescence. In the material labeling method, the separation of the fluorescent substance from the surface of the HAP nanoparticles during the dissolution process of the HAP nanoparticles caused the problem that the dissolution process of the HAP nanoparticles could not be accurately reflected.

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