A Chinese fir forest land cover combustibles decomposing microbial composite bacterial agent and its preparation and application method

A technology of compound bacterial agents and microorganisms, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve the problems of less research and poor pertinence, and achieve the effect of reducing load and maintaining soil fertility

Active Publication Date: 2016-05-11
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the climatic conditions in different regions and the woodlands of different tree species use what kind of microorganisms and their combinations to degrade combustibles. The effect is very different. At present, there are relatively few studies on this type of microbial degradation bacteria agents. Sex is not strong

Method used

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  • A Chinese fir forest land cover combustibles decomposing microbial composite bacterial agent and its preparation and application method
  • A Chinese fir forest land cover combustibles decomposing microbial composite bacterial agent and its preparation and application method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 Composite bacterial agent preparation

[0022] PanusrudisFG-35 was activated to the slant, and cultured at 28°C for 72 hours. BacillussubtilisY13 and BacilluslicheniformisAM-53 were activated to the slant, and cultured at 28°C for 48 hours. Prepare PanusrudisFG-35 bacterial culture: in liquid fungal medium (PD), culture at 30°C, 200rpm for 72h; prepare BacillussubtilisY13 bacterial culture: in liquid bacterial medium (beef extract, peptone), 30°C, 200rpm Cultivate under conditions for 60 hours; prepare Bacilluslicheniformis AM-53 bacterial culture solution: in liquid bacterial culture medium (beef extract, peptone), cultivate for 60 hours at 30°C and 200rpm; The number of live bacteria is: 3.0×10 8 cfu / ml~5.0×10 8 cfu / ml, the number of effective viable bacteria of each bacteria ≧1.0×10 8 cfu / ml. Then add synergist in proportion: K 2 HPO 4 1.00g / L, ZnSO 4 ·7H 2 O0.008g / L, MgSO 4 ·7H 2 O0.5g / L, CuSO 4 ·H 2 O0.007g / L, FeSO 4 ·7H 2 O0.005g, MnSO ...

Embodiment 2

[0023] Example 2 Degradation of fir woodland cover combustibles test in the field

[0024] 1 plot setting

[0025] As a sample plot, a Chinese fir forest in Huangfengqiao Forest Farm, Hunan Province, with a forest age of 30-40 years was selected for research. The size of the sample plot is 50m×50m, and there are 3 standard plots in total. Land cover combustibles spraying plot setting: In each standard plot of 50m×50m, 9 small plots of 1mx1m are randomly selected.

[0026] 2 Degradant spraying and post-sampling

[0027] In mid-April, when the temperature is between 22°C and 30°C, three doses (100ml, 200ml, 400ml) of compound bacterial agents are sprayed into the small sample squares that have been set after rain or on cloudy days. Late sampling: Samples from sprayed and unsprayed areas were collected on the 30th day after spraying, and brought back to the laboratory for air drying. The air-dried samples were dried at 80°C to constant weight, and the content of lignin and cel...

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Abstract

The invention belongs to the technical field of ground cover combustible microbial degradation of Chinese fir forests and particularly relates to a ground cover combustible decomposing microbial compound agent for the Chinese fir forests as well as preparation and application methods thereof. The agent is a liquid agent formed by Panus rudis FG-35 with the preservation number of CCTCC (China Center for Type Culture Collection) NO.M2013106, Bacillus subtilis Y13 with the CCTCC NO.M208264 and Bacillus licheniformis AM-53 with the CCTCC NO.M2012273. According to the invention, aiming at the characteristics of the Chinese fir forests, ground cover combustibles are rapidly and efficiently degraded by utilizing a specific microbial combination, so that inorganic elements are returned to soil, the soil fertility is maintained, the stability of forest productivity is guaranteed, the ground cover combustible load is effectively reduced and the whole process is friendly to environment and free of pollution.

Description

technical field [0001] The invention belongs to the technical field of microbial degradation of combustibles in Chinese fir forest, and in particular relates to a composite microbial agent for decomposing combustibles in Chinese fir forest and its preparation and application method. Background technique [0002] At present, forest fires occur in a wide range of areas all over the world. They are sudden and destructive natural disasters that are difficult to deal with and rescue. They are extremely destructive to forests and cause serious losses. After forest fires, especially moderate and severe fires, energy flow and logistics will be blocked, nutrients will be lost in large quantities, swamps, grasslands and meadows, pests and diseases will occur, and even a vicious circle of forest fires will be caused, which will make the structure of forest ecosystems And the function is destroyed, become the disturbing factor that is not conducive to the balance. In addition, the occu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14C05F17/00C05F15/00C12R1/125C12R1/10C12R1/645
CPCY02W30/40
Inventor 李河朱丹雪刘剑冯杰周国英谭益民刘君昂
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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