Method for realizing secondary purification by using semi-permeable membrane

A secondary purification, semipermeable membrane technology, applied in the preparation methods of peptides, chemical instruments and methods, organic chemistry, etc., can solve the problems of unreported semipermeable membrane secondary purification methods, etc. Improve the effect of enrichment purity and high purity

Inactive Publication Date: 2014-06-11
上海市闵行区知识产权保护协会 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the purity of the finished product obtained by this method is low, and the polypeptide content contained is relatively high.
[0006]In summary, although the separation of antioxidant active peptides has been studied to some extent, there is no report in the literature on the use of semi-permeable membranes for secondary purification

Method used

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Embodiment Construction

[0014] The embodiments of the present invention are described in detail below. This embodiment is implemented on the premise of the technical solution of the present invention, and detailed implementation methods and specific operating procedures are provided, but the protection scope of the present invention is not limited to the following implementation example.

[0015] The secondary purification method using a semi-permeable membrane described in this example includes the following key raw material stages: a mixture of wheat germ and water 1, a composite raw material 2, and an electrophoresis raw material 3.

[0016] In this embodiment, the mixture 1 of wheat germ and water refers to pulverizing the wheat germ at room temperature to obtain a product with a density of 1.01-5.2 grams per cubic centimeter.

[0017] In this embodiment, the composite raw material 2 refers to the raw material prepared by uniformly mixing the above-mentioned mixture 1 of wheat germ and wa...

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Abstract

The invention discloses a method for realizing secondary purification by using a semi-permeable membrane. Key raw materials used in the invention comprise a wheat germ and water mixture 1, a composite raw material 2 and an electrophoresis raw material 3. Anti-oxidative bioactive peptides having a consistent molecular weight can be obtained by using low-cost raw materials through the method, and highly-pure anti-oxidative bioactive peptides can be obtained through the secondary purification by using the semi-permeable membrane.

Description

[0001] technical field [0002] The invention relates to a method for separating antioxidant active peptides in the field of bioengineering, in particular to a secondary purification method using a semipermeable membrane. Background technique [0003] In recent years, more and more attention has been paid to the research and development of the separation of antioxidant active peptides, especially the problem of low-cost and high-purity separation. The traditional isolation of antioxidant active peptides is enriched by filtration, precipitation and other methods. The process is complicated and the purity of the finished product is limited, which is limited in practical application. [0004] Chinese application number CN200810064296.8, application publication number CN101284872, the patent discloses an antioxidant active peptide and its preparation method, the invention uses the microorganism Bacillus natto as the strain, corn gluten powder as the raw material, through liquid ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/26C07K1/34
Inventor 杨星林
Owner 上海市闵行区知识产权保护协会
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