Method for realizing secondary purification by using semi-permeable membrane

Abstract

The invention discloses a method for realizing secondary purification by using a semi-permeable membrane. Key raw materials used in the invention comprise a wheat germ and water mixture 1, a composite raw material 2 and an electrophoresis raw material 3. Anti-oxidative bioactive peptides having a consistent molecular weight can be obtained by using low-cost raw materials through the method, and highly-pure anti-oxidative bioactive peptides can be obtained through the secondary purification by using the semi-permeable membrane.

Description

[0001] technical field [0002] The invention relates to a method for separating antioxidant active peptides in the field of bioengineering, in particular to a secondary purification method using a semipermeable membrane. Background technique [0003] In recent years, more and more attention has been paid to the research and development of the separation of antioxidant active peptides, especially the problem of low-cost and high-purity separation. The traditional isolation of antioxidant active peptides is enriched by filtration, precipitation and other methods. The process is complicated and the purity of the finished product is limited, which is limited in practical application. [0004] Chinese application number CN200810064296.8, application publication number CN101284872, the patent discloses an antioxidant active peptide and its preparation method, the invention uses the microorganism Bacillus natto as the strain, corn gluten powder as the raw material, through liquid ...

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Problems solved by technology

However, the purity of the finished product obtained by this method is low, and the polypeptide content contained is relatively high.

[0006]In summary, although the separation of antioxidant active peptides has been studied to some extent, there is no report in the literature on the use of semi-permeable membranes for secondary purification