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Molecular cloning and application of porcine nr1h4 gene

A technique of gene and genotype, applied in the field of nucleic acid molecules containing porcine gene nucleotides as shown in SEQ ID NO: 1, single nucleotide polymorphism sites

Active Publication Date: 2016-04-27
HUNAN CHUWEIXIANG AGRICUTLTURE CO LTD
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are very few studies on the porcine NR1H4 gene at home and abroad

Method used

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  • Molecular cloning and application of porcine nr1h4 gene
  • Molecular cloning and application of porcine nr1h4 gene
  • Molecular cloning and application of porcine nr1h4 gene

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Embodiment Construction

[0034] The present invention will be further explained below in conjunction with specific examples, but the specific implementation does not limit the present invention in any way.

[0035] Using the human NR1H4 gene sequence (GenBank accession number NM_001206993) as the seed sequence, using BLASTN in GenBank to design specific primers with reference to the porcine EST (ExpressedSequenceTags) with more than 80% homology, and using RACE technology to clone the porcine NR1H4 gene The polymorphism of the full-length cDNA and the NR1H4 gene was designed using comparative genomics methods based on the human NR1H4 gene sequence, amplified with pig genomic DNA as a template, and the amplified fragment was screened for SNP by sequencing, and an A was found at 709 bp The base mutation of / G resulted in PCR-RFLP-MwoI polymorphism, and the distribution of this marker in Chinese and foreign pig breeds was detected by this method.

[0036] Take the longissimus dorsi muscle of 25-day-old S...

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Abstract

The invention belongs to the molecular biological field of molecules of livestock and in particularly relates to molecular clone of a porcine NR1H4 gene and an application. A molecular marker is cloned by the NR1H4 gene, the sequence of which is shown as SEQ ID NO:1. Polymorphism of the NR1H4 gene means that by taking a porcine genomic DNA (Deoxyribonucleic Acid) as template amplification according to a human NR1H4 gene sequence designed primer, SNP (Single Nucleotide Polymorphism) is screened by sequencing an amplified fragment, and genetic typing is carried out by PCR-RFLP (Polymerase Chain Reaction-Festriction Fragment Length Polymorphism), discovering that an A / G basic group mutates at 709bp to lead to PCR-RFLP-Mwo I polymorphism. Conditions of China and foreign countries in China and foreign countries are detected by using the marker. The invention provides a novel molecular marker for porcine marker assisted selection.

Description

technical field [0001] The invention belongs to the technical field of livestock molecular biology, and relates to a nucleic acid molecule comprising porcine gene nucleotides as shown in SEQ ID NO:1. The present invention also relates to the molecular cloning method of the NR1H4 gene shown in SEQ ID NO: 1, the single nucleotide polymorphism site in the polynucleotide sequence and the method for detecting the single nucleotide polymorphism site. Background technique [0002] Pork is the main source of animal protein. As people's demand for meat increases, the requirements for meat quality also increase accordingly. There are genetic and non-genetic factors that affect pork quality. The fat content in pig muscle has a great influence on the meat quality. Generally speaking, the higher the fat content in the muscle, the tenderer the pork quality, and the better the meat quality. Most meat quality traits belong to quantitative traits, which are controlled by minor polygenes. M...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/12C12Q1/68C12N15/11
Inventor 马海明杨虎蒋隽何俊贺长青
Owner HUNAN CHUWEIXIANG AGRICUTLTURE CO LTD
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