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Detection kit for auxiliary diagnosis of coronary heart disease and detection method thereof

A detection kit and auxiliary diagnosis technology, which is applied in the direction of microbial determination/inspection, biochemical equipment and methods, etc., can solve the problems of non-disclosure, etc., and achieve the effects of high accuracy, simple method, and easy operation

Inactive Publication Date: 2014-07-02
NINGBO UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, there are no relevant research reports on kits for detecting the correlation between APOE gene rs7259620 and APOA5 gene rs662799 single nucleotide polymorphism (SNP) and coronary heart disease at home and abroad.

Method used

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  • Detection kit for auxiliary diagnosis of coronary heart disease and detection method thereof
  • Detection kit for auxiliary diagnosis of coronary heart disease and detection method thereof

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Embodiment Construction

[0042] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0043] A detection kit for auxiliary diagnosis of coronary heart disease, the detection kit comprises APOE gene primers for detecting APOE gene rs7259620 single nucleotide polymorphism and APOE gene primers for detecting APOA5 gene rs662799 single nucleotide polymorphism APOA5 gene primer;

[0044] Described APOE gene primer is the nucleotide fragment that comprises following sequence:

[0045] APOE gene-specific upstream primer one: 5'-gcgggcagggcggcTCACTGTGTGGTTTTGCCATTCg-3';

[0046] APOE gene-specific upstream primer two: 5'-gcgggcTCACTGTGTGGTTTTGCCATTCa-3';

[0047] APOE gene reverse sequencing primer: 5'-AACCCGTGGTTCAGCAGCAAGA-3';

[0048] Described APOA5 gene primer is the nucleotide fragment that comprises following sequence:

[0049] APOA5 gene-specific upstream primer one: 5'-gcgggcagggcggcCCAGGAACTGGAGCGAAAGTg-3';

[0050] APOA...

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Abstract

The invention discloses a detection kit for auxiliary diagnosis of coronary heart disease. The detection kit comprises an APOE gene primer for detecting rs7259620 single nucleotide polymorphism of an APOE gene and an APOA5 gene primer for detecting rs662799 single nucleotide polymorphism of an APOA5 gene. The detection method comprises the steps: PCR amplification is carried out on a sample DNA through the APOE gene primer and the APOA5 gene of the detection kit; a melting curve of the PCR amplification product is analyzed by using a fluorescent quantization PCR apparatus; the melting curve is compared with the standard melting curve to compare the Tm value of the PRC amplification product so as to obtain a corresponding genotyping result. The detection kit can detect the coronary heart disease of a subject, and the detection method is simple, high in detection efficiency, and strong in pertinence, so as to facilitate early prevention of the coronary heart disease and provide reference for medication.

Description

technical field [0001] The invention relates to the technical field of auxiliary diagnosis of coronary heart disease, in particular to a detection kit for auxiliary diagnosis of coronary heart disease and a detection method thereof. Background technique [0002] Coronary heart disease, also known as ischemic heart disease (CHD), involves atherosclerosis in the arteries supplying myocardial blood, that is, coronary atherosclerosis leads to narrowing of the vessel lumen or plaque formation or even rupture, complete blockage, limiting Or completely interrupt the blood supply of the myocardium, causing clinically a series of severe myocardial ischemic diseases such as angina pectoris and myocardial infarction. Coronary heart disease is one of the most serious diseases threatening human health today, and it is the main killer of human health. With the rapid development of the economy, the spectrum of diseases worldwide has changed significantly, and cardiovascular diseases have ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q1/686C12Q2600/156
Inventor 叶华丹段世伟周安楠洪青晓李奕润
Owner NINGBO UNIV