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A Method for Analyzing Vein Topological Structure of Xerophyte Broad-leaved Plants

A broad-leaved plant and analysis method technology, applied in the field of leaf vein topology analysis, can solve problems such as inapplicable scientific research, long operation time, and lack of pertinence

Inactive Publication Date: 2016-08-31
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current techniques for extracting veins are mostly used in the production of vein bookmarks and vein flowers. The operation time is long and not targeted, and it is not suitable for scientific research.
In terms of leaf vein data analysis, most foreign researchers use electronic equipment to simulate leaf configuration to obtain leaf vein images, and rarely obtain reliable leaf vein topological structure data for specific species

Method used

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  • A Method for Analyzing Vein Topological Structure of Xerophyte Broad-leaved Plants
  • A Method for Analyzing Vein Topological Structure of Xerophyte Broad-leaved Plants
  • A Method for Analyzing Vein Topological Structure of Xerophyte Broad-leaved Plants

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Collection of experimental materials: Select mature leaves on the sunny side of the top of heather (leathery leaves) and Buddleia (filmy leaves) plants, and fix them with FAA after picking;

[0021] (2) Digestion treatment: heat ultrapure water to 37°C, put in the leaves and continue to heat until boiling. Soak heather in pure water and boil for 10 minutes. Buddleja in pure water soak and boil for 10 minutes. 18 minutes; prepare a sodium hydroxide solution with a mass fraction of 8%, heat and maintain it at 80°C, and soak the leaves boiled in pure water in the sodium hydroxide solution. The time is 35 minutes;

[0022] (3) Leaf vein clearing treatment: Put the boiled leaves into a petri dish filled with clean water, gently brush the upper epidermis and mesophyll of the leaves with a brush, and change the water constantly during the period to ensure the integrity and clarity of the leaf veins;

[0023] (4) Observation and shooting: use filter paper to blot the water...

Embodiment 2

[0026] (1) Collection of experimental materials: Select the mature leaves on the sunny side of the top of the paper-leafed plants—Barrow, Aster and Aster, and fix them with FAA after picking;

[0027] (2) Digestion treatment: Take ultrapure water and heat it to 37°C, put in the leaves, continue to heat until boiling, soak and boil for 12 minutes in pure water, soak and boil Aster glabra and Aster lamina in pure water The total boiling time is 17 minutes;

[0028] (3) Leaf vein clearing treatment: put the boiled leaves into a petri dish filled with clean water, and gently brush off the epidermis with a brush;

[0029] (4) Observation and shooting: Blot the water on the leaf surface with filter paper, put it on a glass slide, add glycerol dropwise, cover the slide, put it under a microscope connected to a computer for observation, and use Motic Images Plus software to take pictures of leaf veins in different parts ;

[0030] (5) Data measurement: Finally, use Motic Images Plus...

Embodiment 3

[0032] (1) Collection of experimental materials: select leathery leaf plants—mature leaves on the sunny side of the top of the Rosa chuanyunensis plant, and fix them with FAA after picking;

[0033] (2) Digestion treatment: heat ultrapure water to 37°C, put in the leaves, continue heating until boiling, soak and boil the leaves in pure water for a total of 8 minutes;

[0034] (3) Leaf vein clearing treatment: Put the cooked leaves into a petri dish filled with water, gently brush off the epidermis with a soft brush, then soak in safranin solution for 24 hours, and then decolorize with alcohol;

[0035] (4) Observation and shooting: Blot the water on the leaf surface with filter paper, put it on a glass slide, add glycerol dropwise, cover the slide, put it under a microscope connected to a computer for observation, and use Motic Images Plus software to take pictures of leaf veins in different parts ;

[0036] (5) Data measurement: Finally, use Motic Images Plus software to mea...

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Abstract

The invention discloses an analysis method of a xerophytic broadleaf plant vein topological structure. The analysis method comprises the steps of heating and boiling plant leaves by using pure water for 5-18 min, softening the leaves, heating and boiling by using a 5-8 percent sodium hydroxide solution for 5-35 min, and separating leaf epidermis from mesophyll; then placing the leaves in a culture dish filled with clear water, and brushing the leaf epidermis and the mesophyll by using a soft brush; then cleaning residual mesophyll by using clear water, sucking water to be dried by using filter paper, observing on a microscope connected with a computer, and shooting a clear image; finally, measuring by using software to analyze a vein density and a ratio of a vein in each stage. According to the analysis method, an image of an experimental plant vein can be clearly shot, data such as the density of the vein in each stage, the ratio of the vein in each stage and the like can be accurately calculated, and a reliable guarantee is provided for relevant research of the vein.

Description

technical field [0001] The invention relates to the analysis of the leaf vein topological structure of plants, in particular to the leaf vein image extraction technology and the leaf vein topological structure analysis method of xeric broad-leaved plants. Background technique [0002] With the deepening and development of plant structure research, leaf veins have gradually become the focus of scientific researchers. Therefore, extracting the complete leaf vein system and obtaining leaf vein-related data has become a key issue for related research. However, the related techniques for extracting veins are mostly used in the production of vein bookmarks and vein flowers. The operation time is long and not targeted, and it is not suitable for scientific research. In terms of leaf vein data analysis, most foreign researchers use electronic equipment to simulate leaf configuration to obtain leaf vein images, and seldom obtain reliable leaf vein topological structure data for speci...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/00
Inventor 孙素静李芳兰包维楷
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S