A colorimetric method for the rapid detection of folate-overexpressing cancer cells

Abstract

The invention discloses a method for quickly detecting folic acid overexpressed cancer cells. The method comprises the following steps: mixing gold nanoparticles with K2PtCl6 and H2PdCl4 in different proportions to prepare an Au@PtPd nanomaterial; performing surface modification on the Au@PtPd nanomaterial by virtue of 6-sulfydryl-hexanol and nitrine-11-alkyl-mercaptan, and coupling Au@PtPd nanomaterial after the surface modification with alkynylated folic acid by adopting a click chemistry technology; and incubating the folate receptor targeted Au@PtPd nanomaterial and cancer cells, connecting to the cell surfaces, catalyzing hydrogen peroxide oxidation color developing agent to perform color change under the existence of the Au@PtPd nanomaterial, so as to enable a color developer solution to be lower than a hydrophilic region of chromatographic paper, air-drying, scanning by virtue of a scanner, and performing colorimetric analysis on presented color. When the concentration of K562 cells is 200-10,000 cells / mL, the K562 cells and the presented color difference have a good linear relation, a linear regression equation is delta I=38.69+0.075c, and a related coefficient R is 0.9931. The method is easy and convenient to operate, quick, low in expense and capable of detecting the cancer cells without professionals.

Description

technical field [0001] The invention relates to a detection method for cancer cells, in particular to a colorimetric method for detecting cancer cells prepared based on AuPtPd nanometer material. Background technique [0002] The occurrence of tumor seriously affects people's quality of life and endangers people's life safety. Accurate, rapid and simple detection of tumor cells and their physiological activities is of great significance for the diagnosis and treatment of diseases. At present, there have been some reports on detection methods, but generally the operation is cumbersome and time-consuming, it is not easy to quantify, the instruments are expensive, and the staff are required to have high professional skills. Therefore, it is of great significance to realize the highly sensitive, fast and simple detection of cancer cells. At present, flow cytometry for cell detection is a new analysis and sorting technology that can quickly detect cells or subcellular structures...

AI Technical Summary

Problems solved by technology

However, due to its expensive analysis cost (instrument price, time consumption of kits, etc.)

At the same time, because such instruments generally have a relatively large volume, their application and popularization are also limited to a certain extent.

[0003] The development of cell detection methods is inseparable from new materials and technologies. Without new breakthroughs in materials and technologies, it is difficult to make research progress in cell detection methods

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