Liquid pholiota adipose strain mixing cultivation process

A liquid strain and process technology, which is applied in the field of the cultivation technology of the yellow umbrella liquid strain mixed with bacteria, can solve the problems of low production efficiency and long production cycle, and achieves shortening the time for filling the bag, early fruiting, and reducing the spread of mycelium. stage effect

Active Publication Date: 2014-07-16
DEZHOU UNIV
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Problems solved by technology

[0004] The present invention provides a kind of culture process of mixing fungus with liquid fungus to solve the problems of long production period and low production efficiency existing in the prior art

Method used

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Embodiment Construction

[0017] A method for cultivating fungus liquid strains mixed with bacteria, adopting the technology of liquid strains mixed with bacteria, inserting the activated strains into a first-stage shake flask for cultivation, and then inserting them into a second-stage shake flask for cultivation to obtain submerged fermented liquid strains species, the process of uniformly mixing the liquid strain and the base material at a ratio of 10ml to 25ml / g, the specific process steps are as follows:

[0018] 1) Activation of strains: Place the dormant strains at room temperature or below normal temperature for 10-20 minutes for activation treatment to prepare for subsequent inoculation work;

[0019] 2) Primary seed shake flask culture: according to the formula 10% potato, 10% carrot, 5% willow stick, 2% glucose, 1% peptone, 2% agar, KH 2 PO 4 0.2%, K 2 HPO 4 0.2%, MgSO 4 0.1%, water 69.5% to make a first-class shake flask culture medium, sub-package in 250ml shake flasks, select the bett...

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Abstract

The invention relates to the technical field of cultivation of edible fungi, and discloses a liquid pholiota adipose strain mixing cultivation process. The liquid strain mixing process is adopted, activated strains are placed into a first-level shake flask to be cultivated and then are placed into a second-level shake flask to be cultivated, then deeply fermented liquid strains are obtained, and the liquid strains and base materials are evenly mixed by the percentage of 10-25ml/100g. The liquid strain mixing method is adopted, in the early growth stage of pholiota adipose, the growth speed of hypha and the contact surface area of fermentation liquid and the base materials are in positive correlation, the liquid strains and the base materials are evenly mixed and make full contact, the hypha can sprout from every part of a fungus bag, the diffusion stage of the hypha is omitted, the bagful time is shortened, fruiting is moved up, and economic benefits are improved.

Description

technical field [0001] The invention relates to the technical field of cultivation of edible fungi, in particular to a process for cultivating fungus mixed with liquid fungi. Background technique [0002] Yellow umbrella, also known as yellow willow mushroom, fatty scale umbrella, etc., is a rare edible fungus with excellent food and medicine and high commercial value. It is rich in nutrients and high in amino acid content, which can refresh the mind and prevent and treat Tumors and Staphylococcus, Escherichia coli, Klebsiella pneumoniae and Mycobacterium tuberculosis infection. The development of yellow umbrella production neither competes with industry for energy or raw materials, nor does it compete with agriculture for land, and has no environmental pollution. It is a new industry with low investment, quick results and high benefits. [0003] But at present, the cultivation of yellow umbrella in my country is still in the initial stage of exploration, and the cultivatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 曾强成沈亮王吉华于家峰
Owner DEZHOU UNIV
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