Liquid pholiota adipose strain mixing cultivation process
A liquid strain and process technology, which is applied in the field of the cultivation technology of the yellow umbrella liquid strain mixed with bacteria, can solve the problems of low production efficiency and long production cycle, and achieves shortening the time for filling the bag, early fruiting, and reducing the spread of mycelium. stage effect
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[0017] A method for cultivating fungus liquid strains mixed with bacteria, adopting the technology of liquid strains mixed with bacteria, inserting the activated strains into a first-stage shake flask for cultivation, and then inserting them into a second-stage shake flask for cultivation to obtain submerged fermented liquid strains species, the process of uniformly mixing the liquid strain and the base material at a ratio of 10ml to 25ml / g, the specific process steps are as follows:
[0018] 1) Activation of strains: Place the dormant strains at room temperature or below normal temperature for 10-20 minutes for activation treatment to prepare for subsequent inoculation work;
[0019] 2) Primary seed shake flask culture: according to the formula 10% potato, 10% carrot, 5% willow stick, 2% glucose, 1% peptone, 2% agar, KH 2 PO 4 0.2%, K 2 HPO 4 0.2%, MgSO 4 0.1%, water 69.5% to make a first-class shake flask culture medium, sub-package in 250ml shake flasks, select the bett...
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