Cultivation technology of liquid fungus mixed with fungus
A technology of liquid strains and technology, which is applied in the field of cultivation technology of liquid strains of yellow mushrooms, can solve the problems of long production cycle and low production efficiency, shorten the time of full bag, produce mushrooms earlier, and reduce the spread of mycelia stage effect
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[0017] A method for cultivating fungus liquid strains mixed with bacteria, adopting the technology of liquid strains mixed with bacteria, inserting the activated strains into a first-stage shake flask for cultivation, and then inserting them into a second-stage shake flask for cultivation to obtain submerged fermented liquid strains species, the process of uniformly mixing the liquid strain and the base material at a ratio of 10ml to 25ml / g, the specific process steps are as follows:
[0018] 1) Activation of strains: Place the dormant strains at room temperature or below normal temperature for 10-20 minutes for activation treatment to prepare for subsequent inoculation work;
[0019] 2) Primary seed shake flask culture: according to the formula 10% potato, 10% carrot, 5% willow stick, 2% glucose, 1% peptone, 2% agar, KH 2 PO 4 0.2%, K 2 HPO 4 0.2%, MgSO 4 0.1%, water 69.5% are made into first-class shake flask culture medium, are sub-packed in 250ml shake flasks, after st...
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