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sil-4r-nap (soluble interleukin-4 receptor-neutrophil-activating protein) fusion gene

A technology that combines genes and genes, applied in the field of genes, can solve problems such as expensive, growth retardation, and decreased bone density, and achieve the effect of easy construction and avoiding complicated processes

Inactive Publication Date: 2014-07-16
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Clinically, inhaled glucocorticoids (GC) and β2 receptor agonists are commonly used to relieve the clinical symptoms of asthma, but they are expensive and long-term use can easily cause many adverse reactions, such as growth retardation, dysphonia, and decreased bone density.

Method used

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  • sil-4r-nap (soluble interleukin-4 receptor-neutrophil-activating protein) fusion gene
  • sil-4r-nap (soluble interleukin-4 receptor-neutrophil-activating protein) fusion gene
  • sil-4r-nap (soluble interleukin-4 receptor-neutrophil-activating protein) fusion gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0026] The fusion gene sIL-4R-NAP can be prepared by conventional methods in the art. The following uses it as an example to illustrate the construction of the vaccine of the present invention, the therapeutic effect of the vaccine, and the protein expression of the fusion gene sIL-4R-NAP. The experimental methods in the following examples are conventional methods unless otherwise specified. The test materials used in the following examples were purchased from conventional biochemical reagent companies unless otherwise specified. % in the following examples, unless otherwise specified, are mass percentages.

[0027] Some materials or instruments:

[0028] Eukaryotic expression vector pcDNA3.1(+) (Invitrogen Company), SPF grade BALB / c mice (Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.),

[0029] Restriction enzymes HindⅢ, BamH Ⅰ, Xho Ⅰ, T4 ligase, Taq DNA polymerase, RNAiso Plus, plasmid mini-prep kit, DNA gel recovery kit, PCR instrument (all purchased from...

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Abstract

The invention relates to the field of genes, and provides a fusion gene which is used for resisting asthma. The gene comprises a fragment (a) coding a soluble interleukin-4 receptor (sIR-4R) and a fragment (b) coding a helicobacter pylori neutrophil-activating protein (HP-NAP). The gene can be connected to a vector to achieve a good effect on resisting asthma.

Description

technical field [0001] The invention relates to the field of genes, in particular to a fusion gene. Background technique [0002] Bronchial asthma, referred to as asthma, is a chronic disease that seriously plagues human health. There are more than 300 million people with asthma worldwide. The incidence of asthma in children in my country has increased by 60% in the past 10 years. At present, there is no cure. Clinically, inhaled glucocorticoids (GC) and β2-receptor agonists are commonly used to relieve the clinical symptoms of asthma, but they are expensive and long-term use can easily cause many adverse reactions, such as growth retardation, dysphonia, and decreased bone density. Asthma is an immune disorder, and many therapeutic drugs are being developed, such as soluble interleukin 4 receptor (sIL-4R), which can block IL-4 signaling and play a certain role in anti-asthma. However, asthma is regulated by a complex signaling network, for example, only blocking IL-4 signal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/62C12N15/63C07K19/00A61K48/00A61P11/06
Inventor 康巧珍傅国汲振余刘鑫王婷曲晓丽王小龙杜明萱
Owner ZHENGZHOU UNIV
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