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Preparation method and applications of S-adenosylmethionine molecular imprinting sensor

A molecular imprinting and sensor technology, which is applied in the preparation and application of S-adenosylmethionine molecular imprinting sensor, can solve the problems of poor regeneration and reversibility, high detection limit, affecting the application of molecular imprinting technology, etc., and achieve improved response , high affinity and selective effects

Inactive Publication Date: 2014-07-30
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the thickness of the imprinted film prepared by the traditional imprinting method is difficult to control, and the high cross-linking degree makes the electron transfer speed and response slow, the detection limit is high, and the regeneration and reversibility are poor, which affects the application of molecular imprinting technology in electrochemical sensors.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Preparation of glassy carbon electrodes modified with gold nanoparticles and carbon nanotubes: the glassy carbon electrodes were sequentially coated with 0.3 μm and 0.05 μm Al 2 o 3 The surface of the powder was polished, then ultrasonically cleaned with high-purity water, dried with argon, and 7 μL of N,N-dimethylformamide dispersion (0.5 g / L) of carbon nanotubes was added dropwise on the surface of the glassy carbon electrode, and placed in an infrared Under the lamp, after evaporating the solvent, soak it in 0.1mol / L chloroauric acid solution for 24 hours, take it out, wash it with deionized water, and dry it naturally to get the nano-gold and carbon nanotube modified glassy carbon electrode;

[0022] (2) Preparation of oxidized carbon nanotubes: In the reactor, add 10mL of concentrated sulfuric acid, 0.5g of carbon nanotubes, and 2.0g of potassium permanganate, ultrasonically disperse for 80min, slowly add 19mL of deionized water, and then stir at 85°C React fo...

Embodiment 2

[0026] (1) Preparation of glassy carbon electrodes modified with gold nanoparticles and carbon nanotubes: the glassy carbon electrodes were sequentially coated with 0.3 μm and 0.05 μm Al 2 o 3 The powder was surface polished, then ultrasonically cleaned with high-purity water, dried with argon, and 5 μL of N,N-dimethylformamide dispersion (0.5 g / L) of carbon nanotubes was added dropwise on the surface of the glassy carbon electrode, and placed in an infrared Under the lamp, after evaporating the solvent, soak in 0.1mol / L chloroauric acid solution for 30 hours, take it out, wash it with deionized water, and dry it naturally to obtain the nano-gold and carbon nanotube-modified glassy carbon electrode;

[0027] (2) Preparation of oxidized carbon nanotubes: In the reactor, add 20mL of concentrated sulfuric acid, 1.5g of carbon nanotubes, and 2.0g of potassium permanganate, ultrasonically disperse for 60min, slowly add 20mL of deionized water, and then stir at 80°C React for 8 hou...

Embodiment 3

[0031] (1) Preparation of glassy carbon electrodes modified with gold nanoparticles and carbon nanotubes: the glassy carbon electrodes were sequentially coated with 0.3 μm and 0.05 μm Al 2 o 3 The surface of the powder was polished, then ultrasonically cleaned with high-purity water, dried with argon, and 10 μL of N,N-dimethylformamide dispersion (0.5 g / L) of carbon nanotubes was added dropwise on the surface of the glassy carbon electrode, and placed in an infrared Under the lamp, after evaporating the solvent, soak in 0.1mol / L chloroauric acid solution for 20 hours, take it out, wash it with deionized water, and dry it naturally to obtain the nano-gold and carbon nanotube-modified glassy carbon electrode;

[0032] (2) Preparation of oxidized carbon nanotubes: In the reactor, add 15mL of concentrated sulfuric acid, 0.5g of carbon nanotubes, and 3.0g of potassium permanganate, ultrasonically disperse for 90min, slowly add 22mL of deionized water, and then stir at 90°C React f...

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Abstract

The invention discloses a preparation method and applications of an S-adenosylmethionine molecular imprinting sensor. The preparation method is characterized by comprising the following steps: firstly modifying a glass-carbon electrode by using nanogold and carbon nano-tubes; successfully researching an imprinting electrochemical sensor with the specific selectivity on the surface of the glass-carbon electrode modified by using the nanogold and the carbon nano-tubes by combining a sol-gel imprinting technology, a layer-by-layer self-assembly method and an electric polymerization method. The S-adenosylmethionine molecular imprinting sensor prepared by adopting the method disclosed by the invention is greatly improved in response, shows the relatively high affinity and selectivity on S-adenosylmethionine forms a sensor which can specifically identify template molecules by being connected with an electrochemical work station and has the advantages of low cost, high sensitivity, good specificity, fastness in detection and repeatability in use.

Description

technical field [0001] The present invention relates to a method for preparing a molecularly imprinted sensor and the technical field of rapid detection application, in particular to a method for preparing a molecularly imprinted sensor for S-adenosylmethionine, specifically based on the molecularly imprinted specific recognition, using S-adenosylmethionine technology used in the detection of pharmaceuticals and biological samples. Background technique [0002] In the methylation reaction in the cell, there is a universal methyl donor—S-adenosylmethionine (AdoMet, SAM). SAM contains an active methyl group, and almost all methylation modifications in the cell The bases all come from the SAM methyl-sulfide high-energy bond. Due to the extensiveness of the methylation reaction, it can be said that SAM is a coenzyme that is second only to ATP in the importance of participating in the reaction in the cell. A small change in the concentration of SAM in the cell will have a signif...

Claims

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Application Information

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IPC IPC(8): G01N27/48G01N27/30
Inventor 李慧芝许崇娟宋桂兰
Owner UNIV OF JINAN
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