Method for preparing quercetin and rhamnose through rutin

A technology of quercetin and rhamnose, applied in the field of extraction of flavonoids, can solve the problems of low product purity not up to international standards, lack of comprehensive utilization of resources, complex and cumbersome process, etc., to achieve complete hydrolysis reaction, The effect of shortening the production cycle and shortening the process flow

Inactive Publication Date: 2014-08-06
罗俊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The purpose of the present invention is to overcome the problems of complex and cumbersome process, long reaction time, low product purity which cannot meet international standards, po

Method used

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  • Method for preparing quercetin and rhamnose through rutin
  • Method for preparing quercetin and rhamnose through rutin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Step a: Collect 10kg (wet weight) of Sophora japonica flower buds, steam the collected Sophora japonica flower buds in 100°C water vapor for 12 minutes to eliminate degradative enzymes, take out the steamed flower buds, dry them in sequence, crush them, and infiltrate them, and use them for percolation A leaching solution containing 18% sodium carbonate, 20% sodium bisulfite, and 30% calcium chloride, the ratio of the leaching solution to Sophora japonica buds (wet weight) is 2.5L: 1kg, and the solution obtained after percolation is filtered to remove slag to obtain a crude extract.

[0030]Step b: Add the natural traditional Chinese medicine clarifier KBT-ZTC to the crude extract obtained in step a. The ratio of the flocculation clarifier to the crude extract is 3.0g:100L. After adding the flocculation clarifier, stir gently to make the flocculation clarifier Distribute evenly in the extract, and then let it stand for 25 minutes to precipitate impurities and remove pre...

Embodiment 2

[0035] Step a: collect 10kg (wet weight) of Sophora japonica flower buds, steam the collected Sophora japonica flower buds in 120°C water vapor for 10 minutes to eliminate degradative enzymes, take out the steamed flower buds, dry them in sequence, crush them, and infiltrate them, and use them for percolation A leaching solution containing 15% sodium carbonate, 30% sodium bisulfite, and 40% calcium chloride, the ratio of the leaching solution to Sophora japonica buds (wet weight) is 3.0L: 1kg, and the solution obtained after percolation is filtered to remove slag to obtain a crude extract.

[0036] Step b: Add the natural traditional Chinese medicine clarifier KBT-ZTC to the crude extract obtained in step a, the ratio of the flocculation clarifier to the crude extract is 2.5g:100L, add the flocculation clarifier and stir gently to make the flocculation clarifier in the crude Distribute evenly in the extract, and then let it stand for 30 minutes to precipitate impurities and re...

Embodiment 3

[0041] Step a: Collect 10kg (wet weight) of Sophora japonica flower buds, steam the collected Sophora japonica flower buds in 115°C water vapor for 13 minutes to eliminate degrading enzymes, take out the steamed flower buds, dry them in the air, crush them, and infiltrate in turn, and use them for percolation A leaching solution containing 30% sodium carbonate, 15% sodium bisulfite, and 30% calcium chloride, the ratio of the leaching solution to Sophora japonica flower buds (wet weight) is 2.0L: 1kg, and the solution obtained after percolation is filtered to remove slag to obtain a crude extract.

[0042] Step b: Add the natural traditional Chinese medicine clarifier KBT-ZTC to the crude extract obtained in step a, the ratio of the flocculation clarifier to the crude extract is 5.0g:100L, add the flocculation clarifier and stir gently to make the flocculation clarifier in the crude Evenly distribute in the extract, and then stand still for 20 minutes to precipitate impurities ...

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Abstract

The invention belongs to the field of flavonoids compound extraction, and particularly relates to a method for preparing quercetin and rhamnose through rutin. The preparation method comprises the following steps: steaming collected sophora flower buds through steam, air-drying, smashing and seeping the steamed sophora flower buds, removing colloid and chlorophyll impurities through flocculation, performing protection complexing treatment through an antioxidant and a complexing agent, adding a mixed acid to adjust the mixture to be acidic, and heating, pressurizing and hydrolyzing to obtain the quercetin; performing impurity filtration and neutralization on hydrolysis mother liquor subjected to quercetin extraction, degrading glucose through glucose degrading enzyme, and performing decoloring vacuum centrifugal concentration and crystallization after a solution is filtered and separated to obtain a product, namely the rhamnose. Under detection of HPLC (high performance liquid chromatography), the content of the quercetin is over 95 percent; all impurity requirements meet an international standard requirement; in a technical process, rutin hydrolysis mother liquor is also subjected to comprehensive treatment, and rhamnose is obtained through production; the production cost of the production is lowered, and the environmental pollution is alleviated.

Description

technical field [0001] The invention belongs to the field of extraction of flavonoids, in particular to a method for preparing quercetin and rhamnose by using rutin. Background technique [0002] Rutin is a kind of flavonoid compound, which is widely distributed in plants in nature, and exists in the roots, stems, leaves, flowers, fruits and seeds of many plants. Some commonly used traditional Chinese medicines, such as Sophora japonica, Rutaceae, Yangmai, Seabuckthorn, Ginkgo, Lycium barbarum, Motherwort, Bupleurum, Prunella vulgaris, Aloe, Eucalyptus leaves and tobacco leaves, etc. contain rutin. One of the important raw materials. [0003] Quercetin belongs to flavonoids and exists in the flowers, leaves and fruits of many plants, mostly in the form of glycosides, such as rutin (rutin), quercetin, hyperoside, etc., which can be obtained by acid hydrolysis quercetin. It has good pharmacological effects such as expectorant and cough, and has a certain antiasthmatic effec...

Claims

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Application Information

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IPC IPC(8): C07D311/30C07D311/40C12P19/02
CPCC07D311/30C07H1/00C07H3/08
Inventor 徐大鹏
Owner 罗俊
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