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Hybridoma cell line and its secreted monoclonal antibody and application of the monoclonal antibody

A hybridoma cell line, monoclonal antibody technology, applied in the direction of analytical materials, microbial-based methods, anti-animal/human immunoglobulin, etc., can solve the problems of ineffective detection of yak milk adulteration, and achieve high efficiency price effect

Active Publication Date: 2017-07-04
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the existing immune technology cannot effectively detect the above-mentioned adulteration of yak milk

Method used

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  • Hybridoma cell line and its secreted monoclonal antibody and application of the monoclonal antibody
  • Hybridoma cell line and its secreted monoclonal antibody and application of the monoclonal antibody
  • Hybridoma cell line and its secreted monoclonal antibody and application of the monoclonal antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] This preparation example is used to illustrate the preparation of hybridoma cells and monoclonal antibodies.

[0064] (1) Preparation of hybridoma cells

[0065] Female Balb / c mice were immunized with traditional cow's milk-specific β-casein according to the method described in Chapter 2 of the "Fine Experiment Guide for Immunology" (Colligan et al., Science Press, 2009). and Milstein ( G., & Milstein, C.1975.Continuous cultures of fused cells secretingantibody of predefined specificity.Nat.256:495-497) described limiting dilution method uses limited dilution method to carry out subcloning, and by ELISA (can refer to "fine editing" Immunology Experiment Guide" (Colligan et al., Science Press, 2009) Chapter 1 Unit 1.1 of the basic protocol section) to screen positive clones, and use yak milk casein to specifically screen positive clones, and finally select positive results It is good and has no cross-reaction to yak milk casein, and the cloned cell line with the best...

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Abstract

The invention discloses a hybridoma cell strain which is characterized in that the preservation number of the hybridoma cell strain is CGMCC NO.8785. The invention further discloses a monoclonal antibody secreted from the hybridoma cell strain, wherein the monoclonal antibody is an antibody resisting specificity Beta-casein in traditional cow's milk. In addition, the invention further discloses application of the monoclonal antibody in identification of milk products doped with traditional milk. According to the invention, the monoclonal antibody generated from the hybridoma cell strain can express higher titer, and has high specificity to the Beta-casein in traditional milk. Therefore, the monoclonal antibody can be used to quickly and effectively identify the adulteration behavior that the traditional milk is doped into rare milk products.

Description

technical field [0001] The present invention relates to a hybridoma cell line, a monoclonal antibody secreted by the hybridoma cell line, and the application of the monoclonal antibody. Background technique [0002] Yak milk is an important economic and food source for the people in Qinghai, my country. Because it is rich in natural nutrients such as conjugated linoleic acid, protein, lactose, milk calcium, lactoferrin, immunoglobulin and vitamins, it is known as "super Therefore, yak milk and its dairy products have received more and more attention in recent years. [0003] However, the yak is a species unique to the Qinghai-Tibet Plateau, mainly distributed in the mountainous areas of central Asia at an altitude of 2500-5500 meters. Due to the constraints of natural ecological conditions and grazing management, the production of yak milk is very limited. This gave rise to the adulteration of traditional milk into yak milk products. [0004] Because yak milk and milk have...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/20C07K16/18G01N33/68G01N33/577C12R1/91
Inventor 任发政任倩然张昊郭慧媛姜鹭
Owner CHINA AGRI UNIV