Bacterial strain antagonistic to pathogenic bacteria of jujube fruit shrink disease and application of bacterial strain
A technology of pathogenic bacteria and strains, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of polluted environment, pesticide residues, etc.
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Embodiment 1
[0057] Example 1 Isolation, screening and identification of jujube fruit shrinkage pathogen antagonistic bacteria Bacillus subtilis STO-45
[0058] 1) Isolation of Bacillus subtilis STO-45, an antagonistic bacterium against Jujube fruit shrinkage pathogen
[0059] The bacterial strain of the present invention is collected from healthy jujube tree root system soil in Yigaiqi Village, Yiliqi Township, Hotan County, Hotan District, Xinjiang.
[0060]1A) Weigh 5g of healthy jujube rhizosphere soil, place it in a sterilized Erlenmeyer flask, soak the soil with 50mL of sterile water, take 1ml of the suspension after slightly shaking at room temperature for 24 hours, and dilute it sequentially by 10-fold gradient dilution method to obtain 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 、10 -8 、10 -9 Concentration gradient dilutions, respectively from 10 -7 、10 -8 、10 -9 Take 0.1mL of the concentration gradient suspension and apply it on the NA medium plate with a sterile ...
Embodiment 2
[0080] The antibacterial activity assay of the fermented liquid of embodiment 2 Bacillus subtilis STO-45 bacterial strain
[0081] 1. Prepare the sterile filtrate of the fermentation broth
[0082] 1A) Pick an activated bacterial lawn of Bacillus subtilis STO-45, inoculate it in a 250mL Erlenmeyer flask filled with 150mL NB medium, culture it with shaking at 28°C and 200r / min for 24h, and obtain the bacterial suspension As seed liquid, NB medium: peptone 10g, beef extract 3g, NaCl 5g, water 1000ml, pH7.0-7.2;
[0083] 1B) Transfer the seed solution to a 250mL Erlenmeyer flask containing 100mL basal medium according to the inoculum amount of 5% (V / V), shake at 28°C and 200r / min, and ferment the culture, wherein the basal medium: glucose 20g, pancreatic Peptone 20g, NaCl 5g, KH 2 PO 4 1g, K 2 HPO 4 2g, water 1000ml, pH7.0-7.2;
[0084] 1C) centrifuging the fermented liquid after 72 hours of fermentation (13000r / min, centrifuged for 10min) to obtain a centrifuged supernatan...
Embodiment 3
[0101] Example 3 Antagonism test of Bacillus subtilis STO-45 on various diseases of jujube and other tree pathogenic fungi
[0102] 1) Pick the lawn of Bacillus subtilis STO-45 cultured on NA medium for 24 hours, put it in a 250mL Erlenmeyer flask filled with 150mL of NB medium, and culture it at a constant temperature of 200r / min at 28°C for 24h to obtain a bacterial suspension As seed liquid, NB medium: peptone 10g, beef extract 3g, NaCl 5g, water 1000ml, pH7.0-7.2;
[0103] 2) Transfer the seed solution to a 250mL Erlenmeyer flask containing 100mL basal medium at an inoculum size of 5% (V / V), shake and ferment at 28°C at 200r / min, wherein the basal medium: glucose 20g, pancreatic Peptone 20g, NaCl 5g, KH 2 PO 4 1g, K 2 HPO 4 2g, water 1000ml, pH7.0-7.2;
[0104] 3) Centrifuge the fermentation broth after 72 hours of fermentation and cultivation (13000r / min, centrifuge for 10min) to obtain the centrifuged supernatant, then take the supernatant and filter it with a 0.45 ...
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