Beggarweed extract, total flavonoids and kaempferitrin separated therefrom, and medicinal uses of beggarweed extract, total flavonoids and kaempferitrin

A technology of extract and kaempferol monomer, which is applied in the field of medicine, and achieves the effect of simple preparation method and obvious effect

Inactive Publication Date: 2014-08-20
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no report on the prevention and treatment of osteoporosis by the extracts of M. chinensis, total flavonoids or monomeric compounds of kaempferin

Method used

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  • Beggarweed extract, total flavonoids and kaempferitrin separated therefrom, and medicinal uses of beggarweed extract, total flavonoids and kaempferitrin
  • Beggarweed extract, total flavonoids and kaempferitrin separated therefrom, and medicinal uses of beggarweed extract, total flavonoids and kaempferitrin
  • Beggarweed extract, total flavonoids and kaempferitrin separated therefrom, and medicinal uses of beggarweed extract, total flavonoids and kaempferitrin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 prepares the extract and kaempferenin monomer compound of Rhizoma chinensis

[0022] 20 kg of the whole herb of M. chinensis, crushed. It is decocted in conventional water, the ratio of solid to liquid is 1:15 (v / v), the extraction time is 2 hours, and the number of extractions is 3 times. The extract was filtered hot, and the filtrates were combined and evaporated to dryness under reduced pressure to obtain 3 kg of extract extract, with an extract yield of 15%. Suspend the extract extract with 1 times the amount of water (3L), and successively add petroleum ether (3L×3), dichloromethane (3L×3), ethyl acetate (3L×3) and n-butanol (3L×3 ) equal-volume extraction, take the n-butanol extract and concentrate it under reduced pressure to form a liquid extract, add 1.5 times of column chromatography silica gel and mix well, load the sample by dry method, wash with dichloromethane:methanol at a ratio of 10:1 to 5:1 Eluate and elute, collect the eluate, evaporate...

Embodiment 2

[0023] Embodiment 2 prepares the total flavonoids of Cyclops chinensis

[0024] 15kg of dried whole grass of Cyclops chinensis, crushed, added 8 times the volume of 95% ethanol, reflux extraction, the extraction time was 3 hours, and the number of extractions was 3 times. The extract is filtered hot, and the filtrates are combined and then concentrated under reduced pressure to a liquid extract, which is the extract of M. chinensis. Add the extract of Lemonia chinensis to the pretreated AB-8 macroporous adsorption resin, wherein the weight ratio of macroporous resin to crude drug of the original medicinal material is 1:1. First eluted with 5 times of column water until colorless, then eluted with 20% ethanol, 30% ethanol, 60% ethanol, 80% ethanol in turn, collected 30-60% of the eluate, and washed After dehydrating and evaporating to dryness, 18.1 g of total flavonoids of M. chinensis were obtained. Taking the kaempferol prepared in Example 1 as a contrast, the total flavono...

Embodiment 3

[0025] Example 3: Preparation of the extract of the leech and the total flavonoids of the leech

[0026] 15kg of dried whole grass of Leaf chinensis, crushed, added 8 times the volume of 80% ethanol, reflux extraction, the extraction time is 3 hours, and the number of extractions is 3 times. After hot filtration, the filtrates are combined and then concentrated under reduced pressure to a liquid extract, which is the extract of M. chinensis. Add the extract of Leedia chinensis to the pretreated polyamide resin of 60-100 mesh, wherein the weight ratio of the resin to the crude drug of the original medicinal material is 1:1. First eluted with water until it was colorless, then eluted with 10% ethanol, 30% ethanol, 60% ethanol, and 80% ethanol in sequence, and collected the eluents eluted with 30% ethanol, 60% ethanol, and 80% ethanol respectively. Taking kaempferol as a control, the total flavonoid content was measured by ultraviolet spectrophotometry (341nm), and the results s...

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Abstract

The invention relates to the technical field of medicines, and relates to applications of a Podocarpium podocarpum extract, and total flavonoids and a kaempferitrin monomer compound separated therefrom in the preparation of medicines or foods for preventing and curing osteoporosis. Dry all grass of Podocarpium podocarpum is extracted and separated as a raw material to obtain the Podocarpium podocarpum extract, the Podocarpium podocarpum extract is used to prepare Podocarpium podocarpum total flavonoids, and the kaempferitrin monomer compound is separated. Results of in vivo and in vitro experiments show that the Podocarpium podocarpum extract, the Podocarpium podocarpum total flavonoids or the kaempferitrin monomer compound has a good anti-osteoporosis bioactivity, so the Podocarpium podocarpum extract, the Podocarpium podocarpum total flavonoids or the kaempferitrin monomer compound can be used for preparing the medicines or foods preventing and treating osteoporosis. The Podocarpium podocarpum extract, the Podocarpium podocarpum total flavonoids or the kaempferitrin monomer compound has a simple preparation method, and provides a new medicine source for the anti-osteoporosis.

Description

technical field [0001] The invention relates to the technical field of medicines, and relates to the application of the extract of the leeches and the total flavonoids and kaempferrin monomer compounds of the leeches isolated therefrom in the preparation of medicines or foods for the prevention and treatment of osteoporosis. This application is a divisional application with the patent application number 201210587958.6. Background technique [0002] Osteoporosis (Osteoporosis, OP) is a common disease frequently-occurring disease. At present, there are about 200 million people in the world suffering from osteoporosis, and its incidence rate has jumped to the seventh among various common diseases in the world. At present, chemical drugs for treating osteoporosis mainly use bone formation stimulators such as estrogen bone resorption inhibitors or androgen, and long-term use has relatively large side effects. Finding effective extracts, effective parts and monomer compounds fro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48C07H17/07A61K31/7048A23L1/29A61P19/10A23L33/00
Inventor 郑承剑秦路平马学琴张巧艳韩婷张宏
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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