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A kind of closed formaldehyde inactivated virus piping system and its application

A formaldehyde inactivation and pipeline system technology, applied in the field of closed formaldehyde inactivation virus pipeline system, can solve the problems of increasing the operation steps of technicians, increasing the degree of pollution risk, and the need for repeated assembly, so as to avoid external pollution and save energy. Production cost, the effect of ensuring sealing

Active Publication Date: 2015-10-07
SINOVAC BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the inactivation operation environment, part of the operation is open production. The pipelines used are relatively simple and scattered, requiring repeated assembly, increasing the operation steps of technicians, and greatly increasing the risk of pollution.
In the process of pipe cleaning, repeated disassembly and assembly are required, and pipes for different purposes are easy to confuse

Method used

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  • A kind of closed formaldehyde inactivated virus piping system and its application
  • A kind of closed formaldehyde inactivated virus piping system and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 closed formaldehyde inactivated virus piping system

[0033] The closed formaldehyde inactivated virus pipeline system provided in this embodiment has a schematic structural diagram as figure 1 and 2 shown. The pipeline system includes a virus liquid tank 1, a pipeline L116, a formaldehyde solution tank 2, a pipeline L217, a T tee 14, a pipeline L318, a peristaltic pump 4, a pipeline L419, a sterilization filter 5, a pipeline L520, an inactivation bucket 3, Balance 6 and magnetic stirrer 7;

[0034] Pipeline L116 extends into the barrel through the barrel cover 11 of the virus liquid barrel, ensuring that one side port of the line L1 is in contact with the bottom of the barrel, and the barrel lid 11 is connected with the air filter 8, and the other end of the line L116 is connected with the line L217 and the line L318 through T Three-way 14-phase connection;

[0035] The pipe L217 extends into the barrel through the barrel cover 12 of the formaldehyde s...

Embodiment 2

[0047] Example 2 Utilize closed formaldehyde to inactivate virus pipeline system to inactivate hepatitis A virus liquid

[0048] Utilize the formaldehyde inactivation virus pipeline system in embodiment 1 to inactivate the hepatitis A virus liquid. The intermediate control and aseptic production operation methods during the inactivation operation are as follows:

[0049] 1. Human diploid cells were cultured in a cell factory, and after being inoculated with hepatitis A virus, cell lysate was added to harvest the hepatitis A virus liquid.

[0050] 2. Obtain a relatively pure hepatitis A virus liquid after primary purification and fine purification of the harvested hepatitis A virus liquid.

[0051] 3. Inactivate the hepatitis A virus liquid and produce the inactivated hepatitis A virus liquid.

[0052] (1) After the inactivation room is cleaned and disinfected, connect the combined pipeline.

[0053] (2) Filtration of hepatitis A virus liquid ( figure 1 ): Connect the virus...

Embodiment 3

[0056] Example 3 Inactivation of poliovirus liquid by using closed formaldehyde inactivation virus pipeline system

[0057] Utilize the formaldehyde inactivated virus pipeline system in Example 1 to inactivate the poliovirus fluid. The intermediate control and aseptic production operation methods during the inactivation operation are as follows:

[0058] 1. After resuscitating and expanding culture of human diploid cells, harvest poliovirus harvest liquid after inoculation of poliovirus seeds and culture.

[0059] 2. Purify the harvested poliovirus fluid through initial purification and fine purification to obtain a relatively pure poliovirus fluid.

[0060] 3. Inactivate the polio virus liquid to produce inactivated polio virus liquid.

[0061] (1) After the inactivation room is cleaned and disinfected, connect the combined pipeline.

[0062] (2) Filtration of polio virus fluid ( figure 1 ): Connect the virus liquid tank 1 into figure 1 In the L1 pipeline, at the same ti...

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Abstract

The invention provides an enclosed formaldehyde virus-inactivating pipeline system. The system comprises a virus liquid barrel (1), a pipeline L1 (16), a formaldehyde solution barrel (2), a pipeline L2 (17), a T-joint (14), a pipeline L3 (18), a peristaltic pump (4), a pipeline L4 (19), a sterilization filter (5), a pipeline L5 (20), an inactivating barrel (3), an electronic balance (6), and a magnetic stirrer (7). The system solves the problems of low formaldehyde virus inactivation efficiency and pollution hazards existing during the aseptic production process due to the diffused pipelines and repeated operation, improves the inactivation efficiency during the virus inactivation process and the control level of aseptic operation, and increases the qualified rate of vaccines.

Description

technical field [0001] The invention relates to a virus inactivation process, in particular to a closed formaldehyde inactivation virus pipeline system and its application. Background technique [0002] In the inactivation process of vaccine production, after the purified virus liquid is sterilized and filtered, it needs to be inactivated by injecting a certain concentration of formaldehyde solution to obtain antigens with stable protein structure and high immunogenicity for the final preparation of vaccines. product. In the virus inactivation process, using a certain concentration of formaldehyde solution to inactivate the virus is the most commonly used method. During the implementation of the method, the factors that greatly affect the fixation of the antigen surface protein by formaldehyde mainly include: the amount of formaldehyde solution added, the flow rate and the stirring of the virus solution. Adding too little or too much formaldehyde solution to the virus solu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12M1/04C12M1/02C12N7/06
CPCC12M27/02C12M37/00
Inventor 张健董兴华张颖黄金凤李静尹卫东
Owner SINOVAC BIOTECH
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