Plant cultivation method and plant application to make it contain salidroside

A technology of salidroside and cultivation method, applied in the field of biotransformation, can solve the problems of low salidroside yield, shortage of resources, complex chemical synthesis methods, etc., and achieve the effects of protecting the liver, enhancing immunity, and protecting cardiovascular

Inactive Publication Date: 2016-05-04
HUNAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to provide a plant cultivation method and plant application to make it contain salidroside, so as to solve the problem of resource shortage caused by the low yield of salidroside and complicated chemical synthesis methods

Method used

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  • Plant cultivation method and plant application to make it contain salidroside
  • Plant cultivation method and plant application to make it contain salidroside
  • Plant cultivation method and plant application to make it contain salidroside

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment one, get Convolvulaceae water spinach, add tyrosol in the water body of its growth, make its initial concentration be 10mmol / L, detect the concentration of tyrosol once every 3~5 days, when down to 8mmol / L (80% of the initial concentration), add tyrosol to make the concentration reach 10mmol / L, and stop adding tyrosol when culturing until 5 days before harvest. After harvesting, the salidroside content (fresh weight) in water spinach can reach more than 0.20%.

[0047] Salidroside detection method: adopt high-performance liquid chromatography, take 0.5g of the sample to be tested, add 25ml of 50% methanol, weigh it, heat and reflux for 30 minutes, let it cool, make up the lost weight, filter, and use it as a test sample. Test solution. Take another salidroside reference substance, add 50% methanol to make a solution containing 0.05mg per 1ml, and use it as the reference substance solution. Octadecylsilane bonded silica gel is used as filler; methanol-water ...

Embodiment 2

[0048] Embodiment 2, get Compositae lettuce lettuce seeds, soak the seeds in clear water, sow in the sandy soil of the cultivation tray, keep moisture, after the cotyledons grow 2cm from the seeds, spray the seedlings with 1 / 10 concentration nutrient solution until 2 pairs of true leaves grow Afterwards, move in the nutrient solution of 1 / 2 concentration and cultivate, monitor the EC value (conductivity) of culture medium every day, when EC value drops to half of initial EC value, change full concentration nutrient solution culture (add 8mmol / L tyrosol), after the same when the EC value drops to half, just replace the full-concentration nutrient solution containing tyrosol, and harvest when the seedling reaches a height of more than 20cm, measure the content of salidroside, and its content (fresh weight) can reach up to 0.3% or more.

[0049] The nutrient solution adopts Hoagland’s formula, which is as follows: calcium nitrate tetrahydrate 945mg / L, potassium nitrate 607mg / L, ...

Embodiment 3

[0050] Embodiment three, get Compositae plant lettuce seedling, cultivate according to the conventional soilless culture method, after growing 3~5 true leaves, add tyrosol in the culture solution so that its concentration is 5mmol / L, every 3~5 leaves 15 days (the initial interval is 15 days, then gradually shorten the interval to 3 days) add tyrosol to the culture medium to make its concentration 5mmol / L, stop adding tyrosol 3 days before harvesting, so that the tyrosol can be converted completely, harvest After the lettuce, the content of salidroside was measured according to the above method, and its content (fresh weight) can reach more than 0.15%.

[0051] Commonly used green leafy vegetable nutrient solution formula is as follows: calcium nitrate tetrahydrate 1260mg / L, potassium sulfate 250mg / L, ammonium dihydrogen phosphate 350mg / L, magnesium sulfate heptahydrate 537mg / L, ammonium sulfate 237mg / L, boric acid 2.86mg / L, borax 4.5mg / L, manganese sulfate tetrahydrate 2.13mg...

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Abstract

The invention provides a salidroside contained plant cultivation method and plant applications. The salidroside contained plant cultivation method comprises adding exogenous butyl alcohol into plant growing water or culture solution, enabling the initial concentration of the plant growing water or culture solution to be 0.1 to 100 mmol per liter; adding the butyl alcohol as supplement or not with interval of certain time; enabling the butyl alcohol to be converted into the salidroside under the function of related enzymes in cells after being absorbed by a plant; accordingly enabling the plant to obtain the physiological pharmacological activity as the salidroside. The plant can be directly used, processed into health food, medicine, food additives and the like or be served as raw materials for extraction of the salidroside.

Description

technical field [0001] The invention relates to the technical field of biotransformation, in particular to a plant cultivation method and plant application for making it contain salidroside. Background technique [0002] Salidroside (salidroside) is the most important medicinal ingredient of Crassulaceae (Crassulaceae) Rhodiola (Rhodiola L.) plant, has anti-fatigue, enhance immunity, protect cardiovascular, anti-hypoxia, anti-aging, protect Nerve cells, liver protection, anti-oxidation, anti-tumor, anti-radiation and other specific effects. It has extremely important application value in military, aerospace, sports and health care medicine. In recent years, there are more and more types of products such as pharmaceuticals, beverages, food and cosmetics produced with it as the main raw material, and people's demand for salidroside is increasing. [0003] Rhodiola plants are the main source of extracting salidroside, but wild resources are scarce, and the content of salidros...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G31/00A01G7/06A23L33/00A23L33/105C07H15/18C07H1/08A61K36/39A61K36/31A61K36/81A61K36/28A61P9/10A61P25/28A61P9/00A61P39/00A61P37/04A61P25/00A61P1/16A61P39/06A61P35/00A61P17/16
Inventor 谭朝阳张倩彭菲于静崔培梧鲁耀邦林艳
Owner HUNAN UNIV OF CHINESE MEDICINE
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