Unlock instant, AI-driven research and patent intelligence for your innovation.

Application of pseudoaltermonas extracellular polysaccharide in inhibition of proliferation of breast cancer cells

A technology of pseudoalteromonas and breast cancer cells, which is applied in the field of functional sugars and can solve the problems of easy detachment of cells and loose connections between cells

Active Publication Date: 2014-08-27
山东博德医药研究院有限公司
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the mammary gland is not an important organ to maintain human life activities, breast cancer in situ will not cause life-threatening, but because breast cancer cells have lost the characteristics of normal cells, the connection between cells is loose, and cells are easy to fall off

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of pseudoaltermonas extracellular polysaccharide in inhibition of proliferation of breast cancer cells
  • Application of pseudoaltermonas extracellular polysaccharide in inhibition of proliferation of breast cancer cells
  • Application of pseudoaltermonas extracellular polysaccharide in inhibition of proliferation of breast cancer cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0033] The preparation steps of the exopolysaccharide (PEP):

[0034] (1) After activating Pseudoaltermonas sp. S-5 with the strain preservation number of CGMCC NO.3433, inoculate it in Zobell2216E liquid medium, cultivate it at 9°C for 24 hours, and inoculate it at 1 wt%. Zobell2216E liquid medium is expanded to cultivate, and the strains can be multiplied;

[0035] (2) by the inoculum amount of 10wt% by the multiplication bacterial classification that step (1) makes, be inoculated in the fermentation medium in the liquid fermentation tank, after adding the sterilized soybean oil of fermentation medium weight 0.3% in the fermentation medium , 7-9°C aerobic stirring culture for 50h, stirring speed 160-170r / min, to obtain a liquid fermentation broth;

[0036] (3) Centrifuge the liquid fermentation broth prepared in step (2) for 15min at 12000r / min, and collect the supernatant;

[0037] (4) Concentrate the supernatant obtained in step (3) to 1 / 4 of the original volume by rotar...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention relates to an application of pseudoaltermonas extracellular polysaccharide in inhibition of proliferation of breast cancer cells. The molecular weight of the pseudoaltermonas sp. S-5 extracellular polysaccharide is 397 kDa; the pseudoaltermonas sp. S-5 extracellular polysaccharide is composed of mannose, glucose and galactose at the mole ratio of 4.8:50.9:44.3. The pseudoaltermonas sp. S-5 extracellular polysaccharide disclosed by the invention can inhibit the proliferation of MDA-MB-231 cells in vitro and inhibit the growth of tumor cells, and the inhibition is achieved mainly by using the pseudoaltermonas sp. S-5 extracellular polysaccharide to induce apoptosis of the MDA-MB-231 cells, so the extracellular polysaccharide has the application prospect in preparation of medicament for inhibiting the proliferation of the MDA-MB-231 cells.

Description

technical field [0001] The invention relates to the application of a pseudoalteromonas exopolysaccharide in inhibiting the proliferation of breast cancer cells, and belongs to the technical field of functional sugars. Background technique [0002] In recent years, the problem of environmental pollution has become increasingly prominent, people's immunity has gradually decreased, and the incidence of malignant tumors has become more and more frequent, and its incidence has gradually increased. Malignant tumors have become the number one killer that threatens human health and are serious diseases that endanger life. At present, tumors can be treated by surgery, radiotherapy, and chemotherapy, but surgery cannot remove metastatic tumors. Radiotherapy and chemotherapy have serious side effects on the human body. Some anti-tumor drugs may affect liver function and some may affect digestion. System, nausea, vomiting and other symptoms, and some can cause neurotoxicity. Therefore...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P35/00A61P15/14
Inventor 陈靠山石磊郭默然于志丹樊恒达
Owner 山东博德医药研究院有限公司