A preparation method of β-cyclodextrin-immobilized cellulose membrane for chiral resolution of tryptophan
A technology of chiral separation and cellulose membrane, which is applied in the field of preparation of tryptophan chiral separation membrane, can solve problems such as the complexity of the preparation process, and achieve the effects of simplifying the preparation process, enhancing stability, and saving production costs
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[0006] Below in conjunction with implementation case, illustrate feature of the present invention:
[0007] Firstly, the β-cyclodextrin-immobilized cellulose membrane was prepared through the following steps, and then the resolution performance of the membrane was characterized and evaluated.
[0008] The steps for preparing β-cyclodextrin-immobilized cellulose membrane are as follows:
[0009] (1) Membrane pretreatment: soak a 0.22 μm cellulose filter membrane in 60 mL of pure water for 1 hour, pour out the pure water, and use the filter membrane for later use.
[0010] (2) Oxidation reaction: put the filter membrane into a beaker, add periodate solution (0.25mM, 5mL), and place it in a constant temperature shaker (25°C, 150r / min) for 2h.
[0011] (3) Cross-linking immobilization reaction: After shaking at a constant temperature for 2 hours, pour out the periodate solution in the beaker, wash it with pure water for 3 times, and put the spare sodium sulfate (0.7M), sulfuric a...
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