Injection molding die and method for PCR (polymerase chain reaction) plates

Abstract

The invention relates to the technical field of PCR (polymerase chain reaction) plate injection molding, and particularly relates to an injection molding die and method for PCR (polymerase chain reaction) plates. The injection molding die comprises an upper die and a lower die, wherein a PCR plate cavity with a panel cavity and multiple reaction tube cavities is formed between the upper die and the lower die, the upper die is provided with multiple plastic inlets, and the bottom of each plastic inlet is respectively communicated with the upper end of the panel cavity; in the process of injection molding, a molten plastic raw material is injected into the PCR plate cavity from the plastic inlets at the upper end of the panel cavity, so that the plastic raw material is filled in the PCR plate cavity so as to form a PCR plate, a water tap of a PCR plate is located on the inner side of a panel, and no water tap is arranged on a reaction tube, thereby improving the surface quality and performance of products; the reaction tube is smooth and neat in surface, stable in size and good in pervious-to-light effect, so that the reaction conditions of samples in the reaction tube can be observed well, thereby improving the detection effect of products; the die needs relatively less plastic inlets, therefore, the structure of the die is simplified, and the production efficiency is high; and water gaps of products are also less, therefore, the die is low in production cost, and raw materials are saved.

Description

Technical field: [0001] The invention relates to the technical field of PCR plate injection molding, in particular to a PCR plate injection molding mold and a method thereof. Background technique: [0002] At present, in the process of molecular biology research and medical clinical diagnosis, PCR reaction technology is indispensable. The PCR reaction is the polymerase chain reaction (polymerase chain reaction, PCR), which is a method for rapidly amplifying a specific gene or DNA sequence in vitro, so it is also called the in vitro amplification method of genes. On both sides of the DNA fragment to be amplified and two oligonucleotide primers complementary to the two sides, after several cycles of denaturation, annealing and extension, the DNA is amplified 2 square times. For batch amplification, PCR reaction plates are commonly used in PCR reactions, see attached Figure 1~3 As shown, the PCR reaction plate includes a panel 1' and a plurality of reaction tubes 2'. The PCR ...

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Problems solved by technology

[0003] When the existing PCR reaction plate is injection molded, generally the PCR reaction plate is inverted and carried out injection molding. The injection molding mold includes an upper mold and a lower mold matched with the upper mold. The lower mold is arranged on the lower end surface of the upper mold, and the upper mold A PCR plate cavity is formed between the lower mold and the PCR plate cavity has a panel cavity 5' and a plurality of reaction tube cavities 6' inverted on the upper end of the panel cavity 5'. The top of the reaction tube cavity 6' is respectively provided with a glue inlet 7', and the raw materials are injected into the PCR plate cavity through each glue inlet 7' at the same time, but the bottom of each reaction tube of the PCR plate produced in this way will form a nozzle , the surface of the bottom of the reaction tube is not smooth, uneven, and the size is unstable, which affects the penetration effect of light, is not conducive to observing the reaction of the sample in the reaction tube, and affects the detection effect. In addition, the injection molding mold needs more glue inlets, and the mold structure Complicated, low production efficiency, high production cost, waste of raw materials

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