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Method for judging carbon source factor capable of improving activity of soil microorganisms based on Biolog micro-plate and microcalorimetric method

A technology of soil microorganisms and microcalorimetry, applied in the direction of material thermal development, color/spectral characteristics measurement, etc., can solve the problems of lack of research, inability to explain the reasons for changes or differences in detail, and inability to reflect the actual situation, etc., to enhance soil fertility , maintain sustainable development, and improve the effect of microbial activity

Inactive Publication Date: 2014-09-10
INST OF SOIL SCI CHINESE ACAD OF SCI
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Problems solved by technology

However, different technologies have their own advantages and disadvantages. For example, Biolog only indicates a small number of microorganisms suitable for growth on microplate media, that is, the metabolic activity is measured by the response of microbial communities to selective media or stimuli, which cannot reflect the actual situation; Isothermal microcalorimetry can measure the overall activity of soil microorganisms, but it is a non-specific method that cannot explain the reasons for changes or differences in detail in complex reaction systems, and needs to be linked with specific analytical methods
There have been many reports on the effects of Biolog or microcalorie on soil microbial activity, but the research on the organic combination of these techniques to explore the dominant factors affecting microbial activity is relatively scarce

Method used

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  • Method for judging carbon source factor capable of improving activity of soil microorganisms based on Biolog micro-plate and microcalorimetric method
  • Method for judging carbon source factor capable of improving activity of soil microorganisms based on Biolog micro-plate and microcalorimetric method
  • Method for judging carbon source factor capable of improving activity of soil microorganisms based on Biolog micro-plate and microcalorimetric method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Taking the soil in Zhucheng, Shandong Province as the research object, the Biolog Eco microplate and microcalorimetric technology were used to compare the differences in soil microbial carbon source metabolism and heat metabolism under the conditions of no fertilization, inorganic fertilizer application, and organic fertilizer application, and to study the effects of soil microbial activated carbon. source factor.

[0015] The experiment consisted of three treatments, each with three replicates. Experimental group 1: treatment with inorganic fertilizer; experimental group 2: treatment with organic fertilizer; control group: treatment without fertilization.

[0016] Biolog Eco Microplate Experiment

[0017] Weigh 10g of soil and put it in 90mL of sterile water. -1 Shake for 30 min, then dilute 1000-fold with sterile water, and inoculate onto BIOLOG microplate (Hayward, CA, USA) with 150 μL per well, and inoculate 3 replicates on the same microplate. Incubate at a cons...

Embodiment 2

[0023] Taking the soil in Fengqiu, Henan Province as the research object, the Biolog Eco microplate and microcalorimetric technology were used to study the differences in carbon source metabolism and heat metabolism capacity of soil microbial communities under different fertilization conditions.

[0024] The experiment consisted of three treatments, each with three replicates. Experimental group 1: treatment with inorganic fertilizer; experimental group 2: treatment with organic fertilizer; control group: treatment without fertilization.

[0025] The Biolog Eco experimental procedure is the same as in Example 1, the factor score coefficients of PC1 and PC2 are shown in Table 1, and the carbon source factor loading diagram of principal component analysis is shown in Table 1. figure 2 As shown in middle b, the relevant carbon sources that cause the differentiation of principal components are shown in Table 2; image 3 Shown in b.

[0026] From the above results, it can be see...

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Abstract

The invention discloses a method for judging a carbon source factor capable of improving activity of soil microorganisms based on a Biolog micro-plate and a microcalorimetric method. The method comprises the following steps: collecting soil samples which are subjected to different fertilization treatments (no fertilizing, fertilizing inorganic fertilizers and fertilizing organic fertilizers), and performing a Biolog Eco micro-plate experiment; performing main component analysis on average absorbance value (AWCD) of Biolog, and distinguishing the carbon source factor causing differentiation; performing a microcalorimetric experiment, and distinguishing effects of different fertilization treatments on thermal metabolic activity of soil microorganisms; respectively adding the carbon source causing the differentiation into unfertilized contrast group soil, and researching influence of different carbon sources on the thermal metabolic activity of the soil microorganisms, so as to judge the carbon source factor capable of improving activity of the soil microorganisms.

Description

technical field [0001] The invention belongs to the field of soil microbial ecology, and in particular relates to a method for judging based on Biolog Eco microplates and microcalorimetry that can improve soil microbial activity factors. Background technique [0002] Soil is a complex system. Soil microorganisms are living organisms in the soil. They participate in the material cycle and energy conversion in the soil, and are important factors that affect the level of soil fertility and the bioavailability of soil nutrient resources. Soil microbial activity is an important indicator to describe the characteristics of soil microbial communities, which can reflect important information of soil conditions. Therefore, studying the effects of different fertilization measures on soil microbial activity has positive significance for optimizing agricultural production management measures. [0003] At present, there are many methods for studying soil microbial activity, such as Biol...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N25/20
Inventor 林先贵徐江兵冯有智
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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