Method for detecting terpene lactones in ginkgo biloba extract
A technology of terpene lactone and detection method, which is applied in the field of detection of terpene lactone in ginkgo biloba extract, can solve problems such as not being able to meet the determination of ginkgo biloba lactone, achieve convenient sample processing methods, shorten test period, cost saving effect
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Embodiment 1
[0034] Embodiment 1: Applicability test of the ginkgo leaf terpene lactone detection system of the present invention
[0035]Take the reference substance solution in Comparative Example 1, and sample it for detection. Analytical column: C18 column, flow rate 0.7mL / min, column oven temperature 30°C. Evaporative light scattering detector conditions: drift tube temperature 100°C, carrier gas pressure 2.5bar, gas flow rate 2.7L / min. Mobile phase conditions: organic phase methanol plus water phase, the volume ratio of methanol to water changes from 25:75 to 65:35; gradient elution program
[0036] time (minutes)
[0037] The peak order of ginkgolides is bilobalide B, ginkgolide G, ginkgolide A, and ginkgolide B. The peak shape is good and the symmetry is good. The peaks were well separated, and the separations all reached above 2.5.
Embodiment 2
[0038] Embodiment 2: implement the detection method of ginkgo leaf extract terpene lactone of the present invention
[0039] Use the reference substance and the test substance used in Comparative Example 1, sample injection detection: respectively accurately draw 5 μL and 10 μL of the reference substance solution, and 10 μL of the test solution, and inject it into the liquid chromatograph for determination. Analytical column: C18 column, mobile phase The flow rate is 0.7mL / min, and the temperature of the column oven is 30°C. Evaporative light scattering detector conditions: drift tube temperature 100°C, carrier gas pressure 2.5bar, gas flow rate 2.7L / min. Mobile phase conditions: organic phase methanol plus water phase, the volume ratio of methanol to water changes from 25:75 to 65:35; the temperature of the column oven is 30°C, and the gradient elution program
[0040] time (minutes)
water box(%)
The organic phase(%)
0
75
25
3
75 ...
Embodiment 3
[0047] Example 3: Quantitative detection of terpenoid lactones in Ginkgo biloba extract
[0048] Reference substance solution is the same as Comparative Example 1;
[0049] Preparation of the test solution: Take about 0.15 g of Ginkgo biloba extract, weigh it accurately, extract 3 times (30 mL, 20 mL, 10 mL) with ethyl acetate at a constant temperature of 40 °C, stir with a magnetic stirrer for 10 min each time, and combine the extractions solution, the ethyl acetate was recovered by a rotary evaporator to dryness, the residue was dissolved in methanol and transferred to a 5mL volumetric flask, added methanol to the mark, shaken well, filtered through a 0.45μm filter membrane, and the subsequent filtrate was obtained.
[0050] Detection method is with embodiment 2;
[0051] Table 3 The results of measuring the content of terpenoid lactones in the Ginkgo biloba extract in the method of the present invention
[0052]
[0053] The results showed that: according to the above ...
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