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Method for detecting terpene lactones in ginkgo biloba extract

A technology of terpene lactone and detection method, which is applied in the field of detection of terpene lactone in ginkgo biloba extract, can solve problems such as not being able to meet the determination of ginkgo biloba lactone, achieve convenient sample processing methods, shorten test period, cost saving effect

Active Publication Date: 2014-09-10
SHANGHAI SINE PROMOD PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the detection time of each sample is as long as 45min. Therefore, it cannot satisfy the rapid determination of ginkgo bilolide, and the detection method needs to be further improved.

Method used

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  • Method for detecting terpene lactones in ginkgo biloba extract
  • Method for detecting terpene lactones in ginkgo biloba extract
  • Method for detecting terpene lactones in ginkgo biloba extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: Applicability test of the ginkgo leaf terpene lactone detection system of the present invention

[0035]Take the reference substance solution in Comparative Example 1, and sample it for detection. Analytical column: C18 column, flow rate 0.7mL / min, column oven temperature 30°C. Evaporative light scattering detector conditions: drift tube temperature 100°C, carrier gas pressure 2.5bar, gas flow rate 2.7L / min. Mobile phase conditions: organic phase methanol plus water phase, the volume ratio of methanol to water changes from 25:75 to 65:35; gradient elution program

[0036] time (minutes)

[0037] The peak order of ginkgolides is bilobalide B, ginkgolide G, ginkgolide A, and ginkgolide B. The peak shape is good and the symmetry is good. The peaks were well separated, and the separations all reached above 2.5.

Embodiment 2

[0038] Embodiment 2: implement the detection method of ginkgo leaf extract terpene lactone of the present invention

[0039] Use the reference substance and the test substance used in Comparative Example 1, sample injection detection: respectively accurately draw 5 μL and 10 μL of the reference substance solution, and 10 μL of the test solution, and inject it into the liquid chromatograph for determination. Analytical column: C18 column, mobile phase The flow rate is 0.7mL / min, and the temperature of the column oven is 30°C. Evaporative light scattering detector conditions: drift tube temperature 100°C, carrier gas pressure 2.5bar, gas flow rate 2.7L / min. Mobile phase conditions: organic phase methanol plus water phase, the volume ratio of methanol to water changes from 25:75 to 65:35; the temperature of the column oven is 30°C, and the gradient elution program

[0040] time (minutes)

water box(%)

The organic phase(%)

0

75

25

3

75 ...

Embodiment 3

[0047] Example 3: Quantitative detection of terpenoid lactones in Ginkgo biloba extract

[0048] Reference substance solution is the same as Comparative Example 1;

[0049] Preparation of the test solution: Take about 0.15 g of Ginkgo biloba extract, weigh it accurately, extract 3 times (30 mL, 20 mL, 10 mL) with ethyl acetate at a constant temperature of 40 °C, stir with a magnetic stirrer for 10 min each time, and combine the extractions solution, the ethyl acetate was recovered by a rotary evaporator to dryness, the residue was dissolved in methanol and transferred to a 5mL volumetric flask, added methanol to the mark, shaken well, filtered through a 0.45μm filter membrane, and the subsequent filtrate was obtained.

[0050] Detection method is with embodiment 2;

[0051] Table 3 The results of measuring the content of terpenoid lactones in the Ginkgo biloba extract in the method of the present invention

[0052]

[0053] The results showed that: according to the above ...

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Abstract

The invention discloses a method for detecting terpene lactones in a ginkgo biloba extract. The ginkgo biloba extract is the raw material and is stirred and extracted through ethyl acetate in a magnetic mode, and a solution is obtained; rotation steaming and concentration are conducted on the solution until the solution is dried, methyl alcohol is used for dissolution, ultrasonic treatment is conducted, the solution serves as a detection sample, and a high performance liquid chromatography-evaporative light-scattering detector is use for analyzing and detecting. According to the method, a detection result is consistent with a detection result in a Chinese pharmacopoeia method, in extracting and separating of the terpene lactones of the ginkgo biloba, the method is more convenient to use and practical. The time for detecting each sample is lower than 12 min (time consumed in the Chinese pharmacopoeia method is 45 min), the detection efficiency is greatly improved, a test cycle is shortened, cost is saved, and the method is economical and environmentally friendly. The method can be used for qualitative and quantitative analysis of the lactones of the ginkgo biloba. It is verified and shown by the methodology that the method is good in separating effect, and the requirements for method linearity, stability, repeatability, the sample adding recycling rate and the like are met. The method is further suitable for detecting preparations with one or more kinds of ginkgo biloba terpene lactones as the raw material, suitable for industrial production and application and large in application value.

Description

technical field [0001] The invention relates to a drug detection method, in particular to a detection method for extracts of traditional Chinese medicines, in particular to a detection method for detecting terpene lactones in ginkgo leaf extracts. Background technique [0002] Ginkgo biloba is the leaf of Ginkgo biloba plant, the main chemical components are flavonoids and terpenoid lactones, which have important medicinal value and market value. Therefore, many Ginkgo biloba series products have been developed on the market. With the progress of society and the development of economy, the importance of ginkgo leaf terpene lactones has attracted more and more attention. Ginkgo biloba terpene lactones are an important component with special structure and significant pharmacological activity found only in Ginkgo biloba. It has not been found in any other plant so far. More importantly, they have unique pharmacological effects and therapeutic value , the common ginkgo leaf ter...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 孙蓉尹小华吕署一李丁肖志勇
Owner SHANGHAI SINE PROMOD PHARMA
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