An experimental method for the influence of trace elements on the growth of algae under different nitrogen and phosphorus conditions

A technology of trace elements and experimental methods, applied in the field of experiments where trace elements affect the growth of algae under different nitrogen and phosphorus conditions, can solve the problems of incompleteness and lack of research, and achieve high accuracy, high sensitivity, and high precision.

Inactive Publication Date: 2016-09-28
XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
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Problems solved by technology

However, the overgrowth of algae and the occurrence of eutrophication are a comprehensive process. It is not comprehensive enough to focus on the unilateral effects of nitrogen and phosphorus nutrients or trace elements. It is necessary to carry out nitrogen, phosphorus nutrients and trace elements Research on the mechanism of synergistic effect, so far, there is still a lack of research on this aspect at home and abroad

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  • An experimental method for the influence of trace elements on the growth of algae under different nitrogen and phosphorus conditions
  • An experimental method for the influence of trace elements on the growth of algae under different nitrogen and phosphorus conditions
  • An experimental method for the influence of trace elements on the growth of algae under different nitrogen and phosphorus conditions

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Embodiment example

[0067] Microcystis aeruginosa was cultivated and tested, and the results were plotted to analyze the influence of different contents of trace elements on the growth of algae under different nitrogen and phosphorus conditions. Table 1 is the pretreatment of Microcystis aeruginosa according to the second step, some measurement results, different nitrogen-phosphorus ratios according to the experimental requirements, and different trace elements Mn 2+ Addition table of some nitrogen source and phosphorus source medium, measured TN=8.1898mg / L, TP=1.4669mg / L, algae cell density M=7.8X10 in Microcystis aeruginosa 7 ; 2+ =9.72ug / L, background value Mn 2+ = 3.24ug / L.

[0068] Table 2, the addition table of nitrogen source and phosphorus source medium under the condition of ensuring the ratio of N and P

[0069]

[0070] Table 3, the addition table of manganese source medium under the condition of ensuring the ratio of N and P

[0071]

[0072] Tables 4, 5, and 6 are the trace...

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Abstract

An experimental method for the influence of trace elements on the growth of algae under different nitrogen and phosphorus conditions. First, a fresh medium is prepared. There is no nitrogen source, phosphorus source and trace elements to be tested in the medium, and then different amounts of N source and P source are put into it. As well as the trace elements to be tested, obtain different N / P ratios and different trace element contents of the medium; then select the experimental algae species and inoculate them into the preset fresh medium containing different nitrogen and phosphorus ratios and different trace element contents middle; then cultivate the algae, measure the algae photometrically, calculate the content of chlorophyll a, then count the algal cell density of the cultivated algae, and finally carry out different nitrogen and phosphorus conditions on the sample according to the measurement results of chlorophyll a and algal cell density. Under the condition that trace elements affect the growth of algae, the present invention can simultaneously measure chlorophyll a and algae cell density in a short time, and quickly reflect the experimental results through the data, which has the advantages of high sensitivity, high precision and high accuracy.

Description

technical field [0001] The invention relates to an experimental method for the influence of trace elements on the growth of algae under different nitrogen and phosphorus conditions. Background technique [0002] Eutrophication is a common problem faced by water bodies, and the overgrowth of algae is the main cause of eutrophication. For the control of algae overgrowth, the key strategy agreed by the international scientific community has always been the scientific control of nutrients such as nitrogen and phosphorus. The U.S. Environmental Protection Agency (USEPA) proposed in the "Nutrient Criteria Technical Guidance Manual: Lakes and Reservoirs" to use the comprehensive trophic state index (Tropic State Index, TSI) to evaluate the eutrophication state of the water body, and the calculation of TSI is related to the total phosphorus (TP ), total nitrogen (TN), chlorophyll a (Chla), transparency (SD) and chemical oxygen demand (COD) indicators are closely related, and it is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/20C12R1/89C12R1/01
Inventor 陈荣罗东程青程喆
Owner XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
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