A kind of pollination method for pomegranate cross breeding

A hybrid breeding and pomegranate technology, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of low hybridization efficiency, large workload of emasculation, reduced fruit setting rate, etc. Avoid the decline of pollen vitality and increase the effect of targeted operation

Active Publication Date: 2016-04-27
INST OF GARDENING ANHUI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The structure of the pomegranate flower is quite special. The hard sepals enclose the flower. In addition, the pomegranate flower has many stamens. A flower usually has two to three hundred stamens. The workload of emasculation during cross-pollination is very large, and the sepals outside the petals It is relatively hard, and the detasseling operation is more difficult, resulting in time-consuming and labor-intensive detasseling work
Traditional pomegranate hybrid breeding mainly adopts the method of emasculation and pollination before flowering of the hybrid female parent. This method often results in low hybridization efficiency and low fruit setting rate due to inconsistent developmental stages of pistils and stamens.
Adopting the method of emasculating and bagging the female flower before opening, and unpacking the pollination when the pistil of the female flower is mature, not only the operation is cumbersome and a waste of manpower, but also the repeated unbagging and bagging operations may cause damage to the style or cause flower damage. fall off, reduce fruit set rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Crossbreeding is carried out with Yushizi as the female parent and Daduzi as the male parent. The specific pollination method is as follows:

[0026] (1) Selection of parents: according to the breeding objectives, select Yushizi with excellent comprehensive traits as the female parent, and use Daduzi with strong resistance, high pollen vigor and large amount of loose pollen as the male parent;

[0027] (2) Collect male parent flowers: On the day of pollination, take some male flowers or neutral flowers whose calyx has cracked and show petals from the Dabenzi plant, and put them in a kraft paper envelope;

[0028] (3) Detasseling of the female parent: select a complete flower whose calyx has cracked and petals are exposed from a robust jade seed plant, and carefully remove the stamens with a crochet hook. When emasculating, be very careful to avoid the crochet needle from hurting the stigma, and pour out all the anthers that have fallen in the calyx tube through the mova...

Embodiment 2

[0033] Crossbreeding is carried out with agate seed as the female parent and Daduzi as the male parent. The specific pollination method is as follows:

[0034] (1) Selection of parents: According to the breeding goal, select the agate seed with excellent comprehensive traits as the female parent, and use the big stupid son with strong resistance, high pollen vigor and large amount of loose pollen as the male parent;

[0035] (2) Collect male parent flowers: before pollination, select some male flowers or complete flowers whose sepals are about to crack from the vigorously growing Dabenzi plant, put them in kraft paper envelopes, and store them in the refrigerator at low temperature;

[0036] (3) Female parent emasculation: Select a complete flower with a cracked calyx and exposed petals from a robust agate seed plant, and carefully remove the stamens with a crochet hook. The active branches will fall into the calyx tube and all the anthers will be poured out;

[0037] (4) Bag...

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PUM

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Abstract

The invention provides a pollination method for pomegranate cross breeding, comprising: (1) selecting parents, (2) collecting male parent flowers, (3) emasculating female parents, (4) bagging, (5) bagging7 -10 days, after the completion of pollination, remove the pollination bag. The present invention adopts the one-time bagging method, the male parent flower is placed in the pollination bag and the pollination bag is covered on the female parent flower, during their continuous development, the pollen of the male parent flower is continuously scattered and scattered on the female parent flower pistil, thereby completing the pollination process, which not only simplifies the operation and saves manpower, but also reduces the damage to the style and the shedding of flowers caused by unbagging and bagging, and effectively improves the hybridization efficiency and hybridization accuracy.

Description

technical field [0001] The invention belongs to the technical field of plant hybrid breeding, in particular to a pollination method for pomegranate hybrid breeding. Background technique [0002] Sexual hybridization is a common and important means of fruit tree breeding, and it is an important way to carry out genetic improvement of fruit trees and cultivate new varieties or new types. The pollination method commonly used in fruit tree hybrid breeding at present is, at the flowering stage of the male parent, picks flowers and prepares pollen, and preserves in low-temperature drying; In order to improve the efficiency of hybridization and increase the accuracy of hybridization, and to avoid selfing of the female parent, there are also bagging after emasculation before the female parent flower is open. pollination process. [0003] Pomegranate is one of the fruit trees widely cultivated in my country, with a long flowering period and a large amount of flowers. The structure...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
Inventor 秦改花徐义流张金云高正辉齐永杰伊兴凯潘海发张晓玲
Owner INST OF GARDENING ANHUI ACADEMY OF AGRI SCI
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