Switch-controlled paper microfluidic chip for detection of clenbuterol-like substances based on SERS

Abstract

The invention belongs to the technical field of food safety inspection and discloses a switch-control-type paper microfluidic chip for detecting clenbuterol hydrochloride based on SERS (surface-enhanced raman scatting). The switch-control-type paper microfluidic chip mainly comprises a chip main body, a first paper tape switch and a second paper tape switch, wherein a main hydrophilic channel uncoated with wax is formed in the fluidic chip main body; N groups of chip units in parallel arrangement are arranged on two sides of the main hydrophilic channel; sample feeding areas, antibody feeding areas, signal detection areas and baffle areas are formed in each group of chip units; the sample feeding areas, the antibody feeding areas and the signal detection areas on two sides of the main hydrophilic channel are respectively communicated through two secondary hydrophilic channels which are uncoated with wax; an included angle between each of the two secondary hydrophilic channels and the main hydrophilic channel is 0 to 90 degrees. Hydrophobic areas coated with wax and hydrophilic areas which are defined by the hydrophobic areas and uncoated with wax are respectively formed in the first paper tape switch and the second paper tape switch; the two paper switches are respectively inserted in the fluidic chip main body at intervals along with dotted lines which are drawn in advance; the paper tape switches are switched on and off by pulling paper tapes. The switch-control-type paper microfluidic chip is used for detecting clenbuterol hydrochloride based on SERS in the site, is high in detection speed, accurate in detection and simple and convenient to operate.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, and relates to a switch-controlled paper microfluidic chip for detecting clenbuterol substances based on surface-enhanced Raman scattering (SERS, surface-enhanced Raman scattering). Background technique [0002] Clenbuterol is a class of hormones, belonging to β-receptor agonists, including clenbuterol hydrochloride, ractopamine, salbutamol and so on. Adding these substances to the feed can increase the animal's weight and make its muscles stronger. Therefore, many farmers use clenbuterol in the breeding industry in order to reduce the use of feed, make meat products go on the market earlier, and reduce costs, even to the point of abuse. After people eat meat products containing β-receptor agonists, symptoms such as muscle tremors, vomiting, nervousness and rapid heartbeat will occur. Therefore, such substances have been listed as prohibited growth promoters in the aquaculture ind...

AI Technical Summary

Problems solved by technology

The first type of method has a high false positive rate, and the second type of method can only be carried out in the central laboratory, and cannot achieve instant on-site detection

Images