A detection method of chlorpyrifos pesticide and its three metabolites in soil
A chlorpyrifos pesticide and detection method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of limited and rare detection items, and achieve the effects of improving extraction efficiency, reducing losses, and simple pretreatment
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Embodiment 1
[0033] In this embodiment, taking black soil as an example, a mixed standard solution of chlorpyrifos pesticide and its three metabolite compounds was prepared, and 0.01, 0.05 and 0.1mg / Kg pesticide standard were added for recovery, and each content level was repeated 3 times.
[0034] Firstly, weigh 5.0 g of a uniformly prepared sample into a 50 mL centrifuge tube, add pesticide standards at the above three concentrations, and repeat 3 times for each concentration level. Then the soil sample containing chlorpyrifos and its three metabolites was vortexed and left for 2 hours to allow the pesticide to fully adsorb to the soil. Then add 0.5 g of anhydrous sodium sulfate and 4.0 g of anhydrous magnesium sulfate, add 20 mL of 1% acetic acid acetonitrile solution, ultrasonically extract for 10 min, and centrifuge at 5000 r / min for 5 min. Then it was concentrated to dryness, and the volume was adjusted to 2 mL with acetonitrile:water (0.1% acetic acid) 90:10. Concentrated and const...
Embodiment 2
[0048] In this example, purple clay was taken as an example to prepare a mixed standard solution of chlorpyrifos pesticide and its three metabolite compounds, and add 0.01, 0.05 and 0.1mg / Kg pesticide standard substances for recovery, and repeat 3 times for each content level.
[0049] Firstly, weigh 5.0 g of a uniformly prepared sample into a 50 mL centrifuge tube, add pesticide standards at the above three concentrations, and repeat 3 times for each concentration level. Then the soil sample containing chlorpyrifos and its three metabolites was vortexed and left for 2 hours to allow the pesticide to fully adsorb to the soil. Then add 0.8 g of anhydrous sodium sulfate and 5.0 g of anhydrous magnesium sulfate, add 25 mL of 1% acetic acid acetonitrile solution, ultrasonically extract for 15 min, and centrifuge at 7500 r / min for 5 min. Then it was concentrated to dryness, and the volume was adjusted to 2 mL with acetonitrile: water (0.1% acetic acid) 40:60. Concentrated and cons...
Embodiment 3
[0063] In this embodiment, taking red soil as an example, a mixed standard solution of chlorpyrifos pesticide and its three metabolite compounds was prepared, and 0.01, 0.05 and 0.1mg / Kg pesticide standard were added for recovery, and each content level was repeated 3 times.
[0064] Firstly, weigh 5.0 g of a uniformly prepared sample into a 50 mL centrifuge tube, add pesticide standards at the above three concentrations, and repeat 3 times for each concentration level. Then the soil sample containing chlorpyrifos and its three metabolites was vortexed and left for 2 hours to allow the pesticide to fully adsorb to the soil. Then add 0.6 g of anhydrous sodium sulfate and 5.0 g of anhydrous magnesium sulfate, add 30 mL of 1% acetic acid acetonitrile solution, ultrasonically extract for 20 min, and centrifuge at 5500 r / min for 5 min. Then it was concentrated to dryness, and the volume was adjusted to 2 mL with acetonitrile: water (0.1% acetic acid) 50:50. Concentrated and consta...
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