Method for analyzing daytime severe cold and flu capsules by high performance liquid chromatography

A high-performance liquid chromatography (HPLC) technology, which is applied in the field of drug analysis to achieve the effects of improving tailing phenomenon, significant precision, and improving peak shape.

Active Publication Date: 2014-10-29
HUMANWELL PURACAP PHARM WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, there is no public report on the simultaneous determination of acetaminophen, phenylephrine hydrochloride, guaiacol glyceryl ether, and dextromethorphan hydrobromide in Ammeyu Soft Capsules

Method used

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  • Method for analyzing daytime severe cold and flu capsules by high performance liquid chromatography
  • Method for analyzing daytime severe cold and flu capsules by high performance liquid chromatography
  • Method for analyzing daytime severe cold and flu capsules by high performance liquid chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Chromatographic conditions:

[0066] High performance liquid chromatography: Waters e2996, PDA detector; Chromatographic column: Agilent SB-C18, 4.6 * 150mm, 5 μ m; Adopt the sodium octane sulfonate solution containing 0.1v% triethylamine (weigh 1.1g octane sulfonate Add 1 mL of triethylamine to 1 L of water, then adjust the pH to 3.0 with phosphoric acid, degas, filter) as mobile phase A, and use acetonitrile as mobile phase B to carry out gradient elution. The gradient elution conditions are shown in Table 1; Flow rate: 1.2ml / min; Detection wavelength: 278nm; Column temperature: 35°C; Injection volume: 20μL;

[0067] Table 1

[0068] time (min)

Mobile phase A (V%)

Mobile phase B (V%)

0

90

10

[0069] 1

90

10

16

50

50

17

90

10

21

90

10

[0070] Experimental steps:

[0071] (1) Preparation of control solution: Accurately weigh 65 mg of paracetamol reference su...

Embodiment 2

[0075] Chromatographic conditions:

[0076] High performance liquid chromatography: Waters e2996, PDA detector; Chromatographic column: Agilent SB-C18, 4.6 * 150mm, 5 μ m; Adopt the sodium octane sulfonate solution containing 0.1v% triethylamine (weigh 1.5g octane sulfonate NaCl was added to 1L water, 1mL triethylamine was added, then the pH was adjusted to 3.5 with phosphoric acid, degassed, filtered) was used as mobile phase A, acetonitrile was used as mobile phase B, and gradient elution was carried out. The gradient elution conditions are shown in Table 2; Flow rate: 1.2ml / min; Detection wavelength: 278nm; Column temperature: 35°C; Injection volume: 20μL;

[0077] Table 2

[0078] time (min)

Mobile phase A (V%)

Mobile phase B (V%)

0

90

10

1

90

10

16

45

55

17

90

10

21

90

10

[0079] Experimental procedure: with embodiment 1.

[0080] Its HPLC spectrum is as Figure 4~5 sho...

Embodiment 3

[0082]Chromatographic conditions:

[0083] High performance liquid chromatography: Waters e2996, PDA detector; Chromatographic column: Agilent SB-C18, 4.6 * 150mm, 5 μ m; Adopt the sodium octane sulfonate solution containing 0.1v% triethylamine (weigh 1.3g octane sulfonate Add 1 mL of triethylamine to 1 L of water, then adjust the pH to 2.5 with phosphoric acid, degas, filter) as mobile phase A, use acetonitrile as mobile phase B, and perform gradient elution. The gradient elution conditions are shown in Table 3; Flow rate: 1.2ml / min; Detection wavelength: 278nm; Column temperature: 35°C; Injection volume: 20μL;

[0084] table 3

[0085] time (min)

Mobile phase A (V%)

Mobile phase B (V%)

0

90

10

1.5

90

10

16

50

50

17

90

10

21

90

10

[0086] Experimental procedure: with embodiment 1.

[0087] Its HPLC spectrum is as Figure 6-8 shown.

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Abstract

The invention discloses a method for analyzing daytime severe cold and flu capsules by high performance liquid chromatography. The daytime severe cold and flu capsules contain acetaminophen, phenylephrine hydrochloride, dextromethorphan hydrobromide and guaifenesin; high performance liquid chromatography analysis conditions are as follows: a chromatographic column adopts an octadecyl silane bonded silica gel column, a sodium octanesulfonate containing 0.1v% triethylamine is used as a mobile phase A, and acetonitrile is used as a mobile phase B. The method can efficiently and simultaneously detect four effective components in the daytime severe cold and flu capsules; furthermore, the method is easy to operate, high in analysis speed, high in repetitiveness and extremely high in specificity; the product quality of a daytime severe cold and flu capsule preparation can be relatively efficiently and comprehensively controlled.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, in particular, the invention relates to a method for analyzing Ammeyu soft capsules by using high performance liquid chromatography. Background technique [0002] Cold is one of the most common respiratory diseases. There are many kinds of medicines for treating colds. Western medicines are very popular because of their relatively transparent safety and quick results. Most cold medicines are compound preparations. For Western medicine, the composition of cold medicine mainly includes the following types of drugs: antipyretic and analgesic drugs, adrenergic receptor agonists, antihistamines, antitussive and expectorant drugs, antiviral drugs Wait. Ammeyu Soft Capsules (English name: Daytime Severe Cold and Flu Capsules) are composed of acetaminophen (325mg), phenylephrine hydrochloride (5mg), guaiacol glyceryl ether (200mg), hydrogen bromide Dextromethorphan (10mg) is a compound preparati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 秦巨波蔡波涛郭云梅沈福星周军伟张群
Owner HUMANWELL PURACAP PHARM WUHAN CO LTD
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