Method for analyzing daytime severe cold and flu capsules by high performance liquid chromatography
A high-performance liquid chromatography (HPLC) technology, which is applied in the field of drug analysis to achieve the effects of improving tailing phenomenon, significant precision, and improving peak shape.
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Embodiment 1
[0065] Chromatographic conditions:
[0066] High performance liquid chromatography: Waters e2996, PDA detector; Chromatographic column: Agilent SB-C18, 4.6 * 150mm, 5 μ m; Adopt the sodium octane sulfonate solution containing 0.1v% triethylamine (weigh 1.1g octane sulfonate Add 1 mL of triethylamine to 1 L of water, then adjust the pH to 3.0 with phosphoric acid, degas, filter) as mobile phase A, and use acetonitrile as mobile phase B to carry out gradient elution. The gradient elution conditions are shown in Table 1; Flow rate: 1.2ml / min; Detection wavelength: 278nm; Column temperature: 35°C; Injection volume: 20μL;
[0067] Table 1
[0068] time (min)
Mobile phase A (V%)
Mobile phase B (V%)
0
90
10
[0069] 1
90
10
16
50
50
17
90
10
21
90
10
[0070] Experimental steps:
[0071] (1) Preparation of control solution: Accurately weigh 65 mg of paracetamol reference su...
Embodiment 2
[0075] Chromatographic conditions:
[0076] High performance liquid chromatography: Waters e2996, PDA detector; Chromatographic column: Agilent SB-C18, 4.6 * 150mm, 5 μ m; Adopt the sodium octane sulfonate solution containing 0.1v% triethylamine (weigh 1.5g octane sulfonate NaCl was added to 1L water, 1mL triethylamine was added, then the pH was adjusted to 3.5 with phosphoric acid, degassed, filtered) was used as mobile phase A, acetonitrile was used as mobile phase B, and gradient elution was carried out. The gradient elution conditions are shown in Table 2; Flow rate: 1.2ml / min; Detection wavelength: 278nm; Column temperature: 35°C; Injection volume: 20μL;
[0077] Table 2
[0078] time (min)
Mobile phase A (V%)
Mobile phase B (V%)
0
90
10
1
90
10
16
45
55
17
90
10
21
90
10
[0079] Experimental procedure: with embodiment 1.
[0080] Its HPLC spectrum is as Figure 4~5 sho...
Embodiment 3
[0082]Chromatographic conditions:
[0083] High performance liquid chromatography: Waters e2996, PDA detector; Chromatographic column: Agilent SB-C18, 4.6 * 150mm, 5 μ m; Adopt the sodium octane sulfonate solution containing 0.1v% triethylamine (weigh 1.3g octane sulfonate Add 1 mL of triethylamine to 1 L of water, then adjust the pH to 2.5 with phosphoric acid, degas, filter) as mobile phase A, use acetonitrile as mobile phase B, and perform gradient elution. The gradient elution conditions are shown in Table 3; Flow rate: 1.2ml / min; Detection wavelength: 278nm; Column temperature: 35°C; Injection volume: 20μL;
[0084] table 3
[0085] time (min)
Mobile phase A (V%)
Mobile phase B (V%)
0
90
10
1.5
90
10
16
50
50
17
90
10
21
90
10
[0086] Experimental procedure: with embodiment 1.
[0087] Its HPLC spectrum is as Figure 6-8 shown.
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