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A kind of immobilization method of petroleum degrading bacteria group

A technology of petroleum degrading bacteria and inoculum, which is applied in microorganism-based methods, biochemical equipment and methods, fixed on or in inorganic carriers, etc., and can solve the problems of inability to achieve simultaneous degradation of petroleum components and long degradation cycle. , to achieve the effect of good immobilization effect, low cost and strong synergy effect

Active Publication Date: 2017-11-03
CHINA PETROCHEMICAL CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been reports on the immobilization technology of petroleum-degrading bacteria. 200910255830.8 discloses a method for immobilizing petroleum-degrading bacteria using reed surface roots as carriers; 201210006059.2 discloses the immobilization method and application of petroleum-degrading bacteria. The immobilized single bacteria, due to the limitations of single bacteria degrading bacteria on the degradation of petroleum hydrocarbons, can not achieve simultaneous degradation of petroleum components, resulting in a longer degradation cycle

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Liquid fermentation of petroleum degrading bacteria

[0021] Preparation of liquid seeds of degrading flora: Take Pseudomonas, Bacillus and Micrococcus and mix them evenly, and inoculate them into 500mL conical triangular shake flasks according to the total inoculation amount of 5%. 300mL, 30°C, 100r / min in air for 12h, until the total number of petroleum-degrading bacteria in the medium is 10 8 CFU / mL or more, the oil-degrading bacterial flora liquid seeds are obtained.

[0022] Wherein, the fermentation medium contains the following components by weight fraction: 8% peptone, 3% beef extract, 8% NaCl, the pH of the medium is neutral, and it is used for sterilization.

[0023] Bacterial flora liquid fermentation: Inoculate the liquid seeds of the degrading flora at an inoculum of 5% in a fermenter equipped with a high-temperature sterilized fermentation medium, and culture at 30°C with air at 100r / min for 12 hours; at an inoculum of 5% Transfer to another fermente...

Embodiment 2

[0028] Preparation of liquid seeds of degrading flora: Take Pseudomonas, Bacillus and Micrococcus and mix them evenly, and inoculate them in a 500mL conical triangular shaker flask according to the total inoculation amount of 10%. 300mL, 37°C, 120r / min air circulation for 36h, until the total number of petroleum-degrading bacteria in the medium is 10 8 CFU / mL or more, the oil-degrading bacterial flora liquid seeds are obtained.

[0029] Wherein, the fermentation medium contains the following components by weight fraction: 10% peptone, 5% beef extract, 10% NaCl, the pH of the medium is neutral, and it is used for sterilization.

[0030] Liquid fermentation of flora: Inoculate liquid seeds of degraded flora at 10% inoculum into fermenters equipped with high-temperature sterilized fermentation medium, culture at 37°C and 120r / min in air for 48 hours; at 10% inoculum Transfer to another fermenter equipped with fresh fermentation medium for transfer culture. After 6 times of trans...

Embodiment 3

[0035] Preparation of liquid seeds of degrading flora: Take Pseudomonas, Bacillus and Micrococcus and mix them evenly, and inoculate them in a 500mL conical triangular shake flask according to the total inoculation amount of 8%. 300mL, 35°C, 110r / min in air for 24h, until the total number of petroleum-degrading bacteria in the medium is 10 8 CFU / mL or more, the oil-degrading bacterial flora liquid seeds are obtained.

[0036] Wherein, the liquid fermentation medium contains the following components by weight fraction: 9% peptone, 4% beef extract, 9% NaCl, the pH of the medium is neutral, and it is used for sterilization.

[0037] Bacteria liquid fermentation: Inoculate the liquid seeds of the degrading flora according to the inoculum amount of 8% in the fermenter equipped with high-temperature sterilized fermentation medium, and cultivate for 24 hours at 35°C and 120r / min in the air; according to the inoculum amount of 8% Transfer to another fermenter equipped with fresh ferm...

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Abstract

The invention discloses a method for immobilizing petroleum-degrading bacteria, belonging to the field of microorganism immobilization. Specifically, the following steps are included: (1) liquid fermentation of petroleum-degrading bacteria; (2) immobilization of petroleum-degrading bacteria on a diatomite / activated carbon composite carrier. The method for immobilizing petroleum degrading bacteria proposed by the present invention has the advantages of low cost, large-scale application and high oil removal efficiency. The immobilized degrading bacteria have strong synergistic effect and high degradation ability, and are suitable for all kinds of oil-containing solid wastes. Biodegradable.

Description

Technical field: [0001] The invention relates to a method for immobilizing petroleum-degrading bacteria, belonging to the field of microorganism immobilization. Background technique: [0002] In recent years, with the increasing demand for oil and its products in various countries around the world, a large amount of oil-containing solid waste generated during the exploration and development of oil and gas resources has been included in the list because it contains petroleum hydrocarbons, heavy metals, and organic matter. National hazardous waste, if discharged directly without treatment, will cause serious harm to the ecological environment. Therefore, oily solid waste pollution has become a major environmental problem that people must face. In oily solid waste treatment technology, biological treatment technology has become an important means of safe treatment and disposal of oily solid waste on site because of its advantages of safety, economy, high efficiency, and no sec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12R1/38
Inventor 单海霞何焕杰刘明国王中华马金刘晓宇位华
Owner CHINA PETROCHEMICAL CORP