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A water-dispersible fluorescent polymer nanoparticle with pH value and mercury ion ratio detection function, preparation method and application

A technology of fluorescent polymers and nanoparticles, applied in chemical instruments and methods, fluorescence/phosphorescence, luminescent materials, etc., to achieve the effects of increasing accuracy, eliminating measurement errors, and simple synthesis routes

Inactive Publication Date: 2016-01-13
HUNAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the existing research on various fluorescent polymer nanoparticle sensors, there are few reports on the combination of pH ratiometric fluorescence sensing and miniemulsion polymerization.

Method used

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  • A water-dispersible fluorescent polymer nanoparticle with pH value and mercury ion ratio detection function, preparation method and application
  • A water-dispersible fluorescent polymer nanoparticle with pH value and mercury ion ratio detection function, preparation method and application
  • A water-dispersible fluorescent polymer nanoparticle with pH value and mercury ion ratio detection function, preparation method and application

Examples

Experimental program
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Embodiment 1

[0026] Embodiment 1: A kind of preparation method of the water-dispersible fluorescent polymer nanoparticle that has pH value and mercury ion ratio detection function, comprises the following steps:

[0027] (1) Miniemulsion polymerization to prepare fluorescent polymer nanoparticles containing EANI.

[0028]Stir the mixture of 0.5g methyl methacrylate, 0.08g AEMH, 0.025g AIBN, 0.075g n-hexadecane and 0.001g EANI on a common magnetic stirrer for 5 minutes to obtain solution a; Ammonium bromide was added to 10ml of water, and stirred on a common magnetic stirrer for 10 minutes to obtain solution b. After mixing the solutions a and b, the pre-emulsion was obtained after stirring at room temperature for 5 minutes. Put the pre-emulsion into a 650W ultrasonic cell pulverizer under an ice-water bath (keep below 20°C), and ultrasonically emulsify for 15 minutes to obtain a stable microemulsion. The mini-emulsion was transferred to a 25ml round bottom flask, and the round bottom fla...

Embodiment 2

[0031] Embodiment 2: A kind of preparation method of the water-dispersible fluorescent polymer nano-particle that has pH value and mercury ion ratio detection function, comprises the following steps:

[0032] (1) Miniemulsion polymerization to prepare fluorescent polymer nanoparticles containing EANI.

[0033] Stir the mixture of 0.5g methyl methacrylate, 0.04g AEMH, 0.025g AIBN, 0.075g n-hexadecane and 0.002g EANI on a common magnetic stirrer for 5 minutes to obtain solution a; Ammonium bromide was added to 10ml of water, and stirred on a common magnetic stirrer for 10 minutes to obtain solution b. After mixing the solutions a and b, the pre-emulsion was obtained after stirring at room temperature for 5 minutes. Put the pre-emulsion into a 650W ultrasonic cell pulverizer under an ice-water bath (keep below 20°C), and ultrasonically emulsify for 15 minutes to obtain a stable microemulsion. The mini-emulsion was transferred to a 25ml round bottom flask, and the round bottom f...

Embodiment 3

[0036] Embodiment 3: A kind of preparation method of the water-dispersible fluorescent polymer nano-particle that has pH value and mercury ion ratio detection function, comprises the following steps:

[0037] (1) Miniemulsion polymerization to prepare fluorescent polymer nanoparticles containing EANI.

[0038] Stir the mixture of 0.5g methyl methacrylate, 0.12g AEMH, 0.025g AIBN, 0.075g n-hexadecane and 0.0015g EANI on a common magnetic stirrer for 5 minutes to obtain solution a; Ammonium bromide was added to 10ml of water, and stirred on a common magnetic stirrer for 10 minutes to obtain solution b. After mixing the solutions a and b, the pre-emulsion was obtained after stirring at room temperature for 5 minutes. Put the pre-emulsion into a 650W ultrasonic cell pulverizer under an ice-water bath (keep below 20°C), and ultrasonically emulsify for 15 minutes to obtain a stable microemulsion. The mini-emulsion was transferred to a 25ml round bottom flask, and the round bottom ...

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Abstract

The invention discloses a water-dispersiblity fluorescent polymer nanoparticle with a ratio detecting function for a pH value and mercury ions as well as a preparation method and an application of the water-dispersiblity fluorescent polymer nanoparticle. The water-dispersiblity fluorescent polymer nanoparticle is prepared by taking methyl methacrylate, 2-aminoethyl methacrylate hydrochloride (AEMH) and 4-ethyoxyl-9-(2-allyl)-1, 8-naphthalimide (EANI) as monomers, taking fluorescein isothiocyanate (FITC) as a surface functionalization reagent and combining a one-step miniemulsion polymerization method and a particle surface modification technology. The water-dispersiblity fluorescent polymer nanoparticle not only can be used for realizing ratio fluorescence detection for the pH value in water, but also can be used for realizing ratio fluorescence detection for mercury ions in the water on the premise that the pH value is determined. Compared with the prior art, the water-dispersiblity fluorescent polymer nanoparticle obtained by the invention can be used for respectively realizing high-sensitivity detection for the pH value in the water and high-selectivity and high-sensitivity quantitative detection for the mercury ions in the water; in addition, the water-dispersiblity fluorescent polymer nanoparticle is simple in synthesis route, convenient to use, suitable for amplified synthesis and actual production application and huge in application prospect in the fields of bio-analytical chemistry, cell biology, medicals and environmental detection.

Description

technical field [0001] The invention relates to the technical fields of material preparation and biochemistry and environmental detection, in particular to a preparation method of water-dispersible fluorescent polymer nanoparticles with the function of detecting pH value and mercury ion ratio. The water-dispersible fluorescent polymer nanoparticle relates to the highly sensitive detection of the pH value in the water environment and the highly selective and highly sensitive quantitative detection of mercury ions in the water. Background technique [0002] It is well known that intracellular pH plays an important role in cellular, enzymatic and tissue activities, including proliferation and apoptosis, ion transport and drug resistance, etc. Small changes in intracellular pH may lead to cellular dysfunction, and have a certain correlation with some common diseases such as cancer and Alzheimer's disease. Therefore, accurate measurement of intracellular pH can provide important ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F220/14C08F220/36C08F2/28C08F8/34C09K11/06G01N21/64
Inventor 陈建王宏李亚侯庆杨吕锟吉添李明莉周智华易平贵
Owner HUNAN UNIV OF SCI & TECH
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