In-vitro enzymatic method potassium determining reagent, preparation method and use method thereof

Abstract

The invention discloses an in-vitro enzymatic method potassium determining reagent including a first reagent and a second reagent, wherein the first reagent and the second reagent are packaged individually and are equal in volume. Concentrations of substances in the first reagent are described as follows: 2.00 mmol / L of an MOPS buffering solution with a pH value being 7.3 and a temperature being 25 DEG C, 1.00g / L of albumin, 5.62 mmol / L adenosine triphosphate, 0.982 mmol / L of EGTA, 32.7 mmol / L of [alpha]-ketoglutaric acid, 38.3 mmol / L of urea, 230 u / L of urea amidohydrolase, 4320 u / L of glutamic acid hydrolase and 0.50 g / L of NaN3. Concentrations of substances in the second reagent are described as follows: 30.9 mmol / L of NaHCO3, 0.845 mmol / L of NADH, 16 mmol / L of MgSO4 and 0.30 g / L of NaN3. Solvents in the first reagent and the second reagent are both pure water. The in-vitro enzymatic method potassium determining reagent can be stored stably in a long period, is high in determination accuracy, is low in cost and solves a problem that the Shigeki method is not available.

Description

technical field [0001] The invention relates to a reagent for determining potassium, in particular to a reagent for determining potassium by enzymatic method in vitro. [0002] The present invention also relates to the preparation method of the above-mentioned in vitro enzymatic potassium determination reagent and the use method of the in vitro enzymatic potassium determination reagent. Background technique [0003] The determination of potassium content in human serum is one of the clinical in vitro diagnostic determination items, and it is also an indispensable item in clinical routine emergency diagnosis. When serum potassium is higher than the upper limit of the reference value, it is more common in adrenal insufficiency. In critically ill patients, if the serum potassium rises to 8mmol / L, the patient may develop ventricular fibrillation; if the serum potassium is lower than the lower limit of the reference value, it is more common in renal tubular disease and severe Di...

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Problems solved by technology

[0036] The present invention is completed in order to solve the deficiencies in the prior art. The purpose of the present invention is to provide the first reagent and the second reagent that can be stored independently for a long time. When the two are mixed and measured, the enzyme activity remains unchanged, and the potassium An in vitro enzymatic potassium determination reagent with high content accuracy and low cost, which solves the problem that the previous Shigeki method cannot be applied

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