A kind of breeding method of the isotype temporary complete maintainer line of recessive genic male sterility in rapeseed

Abstract

The invention discloses a breeding method for a rapeseed recessive genic male sterility homotype temporary complete maintainer line. The method is used for breeding the homotype temporary complete maintainer line of a specific homozygous dual-purpose line; fertile pollens are generated by hybrid sterile plants subjected to stressing treatment under certain high temperature, and the rapeseed recessive genic male sterility homotype temporary complete maintainer line is formed by performing backcross breeding on the fertile pollens and sterile plants in the specific homozygous dual-purpose line. Compared with a conventional breeding method, the breeding method disclosed by the invention has the advantages that the backcross times are obviously reduced, and the breeding time is greatly shortened; the pollens used in each backcross are supplied by the c-type hybrid sterile plants, so that the backcross purpose is definite; the breeding effect and the breeding time are the same as those of a molecule labeling auxiliary breeding method.

Description

Technical field [0001] The invention belongs to the field of selection and breeding of rapeseed lines, and particularly relates to a method for selection and breeding of a recessive genic sterile homotypic temporary complete maintenance line of rape. Background technique [0002] Recessive epistasis nucleus sterility is an important way to utilize the heterosis of rapeseed. This method of utilization has obtained the national technology invention patent (ZL97125803.1) in 2003. This patented method has successfully achieved the homozygous recessive nucleus sterility of rapeseed. The dual-use line, the temporary complete maintainer line and the restorer line are three-line matching seed production. In seed production, the sterile plants in the homozygous dual-use line and the temporary complete maintainer line are first used to produce the fully sterile line, and then the fully sterile line and The restore line produces hybrids. [0003] In the breeding process, the temporary comple...

AI Technical Summary

Problems solved by technology

The uniformity of three-cross is not as good as that of single-cross, and it is easy to be considered by seed management companies and farmers as poor seed purity and unacceptable in production.

[0005] There are many methods for the selection of isotype temporary complete maintainer lines. At present, there are two main strategies: one is for the existing homozygous dual-purpose lines, or to breed homozygous dual-purpose lines first, and then use the sterile lines in the homozygous dual-purpose lines. The recurrent parent is backcrossed with the temporary complete maintainer line. Generally, more than 8 generations of backcrossing can produce the isotype temporary complete maintainer of the homozygous dual-purpose line. It is characterized by being suitable for breeding a specific homozygous dual-purpose line. According to the fertility of the progeny of each backcross, the maintenance rate of the selected individual plants can be measured, and the selection purpose is strong; the disadvantage is that the selection time is too long, requiring more than 20 generations. The technical route is shown in figure 1 The second is to construct a population with various fertility-related genotypes and meet the breeding objectives, and simultaneously select and breed homozygous dual-purpose lines and isotype temporary complete maintainers, which are characterized by homozygous dual-use lines and isotype temporary complete maintainers. The maintainer line can be bred at the same time, with high efficiency; the difficulty is that during the breeding process, it needs to be tested by test cross or molecular marker technology to ensure that the target genotype cannot be lost. In practice, molecular marker technology is often used to greatly improve Selection efficiency, but the workload of in-house molecular labeling is very large

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