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Application of beta-arrestin-1 in preparing medicine for preventing or treating myocardial infarction

A technology of inhibiting protein and myocardial infarction, which is applied in the field of medicine, can solve the problems that there is no protective effect of β-arrestin, and achieve the effect of high safety

Active Publication Date: 2014-11-26
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Currently, there are no reports of β-arrestin's protective effect on myocardial infarction

Method used

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  • Application of beta-arrestin-1 in preparing medicine for preventing or treating myocardial infarction
  • Application of beta-arrestin-1 in preparing medicine for preventing or treating myocardial infarction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Effect of β-arrestin-1 gene on infarct size after myocardial infarction.

[0021] β-arrestin-1 knockout mice were purchased from KOMP, Cat. No. CSD47583;

[0022] β-arrestin-1 transgenic mice were constructed by Shanghai Nanfang Model Biotechnology Development Co., Ltd. and bred and cultivated by themselves;

[0023] 1. Construction of a mouse myocardial infarction model

[0024] First put the mouse in a small box connected to the anesthesia machine, take it out after the mouse is anesthetized, and fix it on the operating table with adhesive tape. And put its mouth in the anesthesia tubule with isoflurane, wipe the right chest of the mouse with alcohol cotton ball, and cut a small opening (cut its skin) near the heart with scissors. Then, hemostats were used to open the thoracotomy at 4 intercostal spaces, and the chest was pressed immediately to squeeze out the heart and make it "stuck" in the incision. No chest leak. Ligate the left coronary artery with...

Embodiment 2

[0028] Example 2: Effect of β-arrestin-1 gene on left ventricular ejection fraction and left ventricular short-axis shortening rate after myocardial infarction

[0029] Mouse Cardiac Ultrasound:

[0030] VisualSonics Vevo2100 was used to detect the ejection fraction and short-axis shortening rate of left ventricle of mice. First anesthetize the mouse with 3% chloral hydrate, then apply depilatory cream on its right chest, and wipe off the hair on its right chest with a dry cotton ball after 5 minutes. Then apply ultrasonic lubricant and detect with MS-400 frequency 30MHz probe. In this experiment, two-dimensional and M-mode ultrasonic diagnostic equipment (Vevo2100, Visual Sonics) were used.

[0031]Experimental results: 10 animals in each group were tested for cardiac function 1.5 months after myocardial infarction. The results showed that the left ventricular ejection fraction and left ventricular short-axis shortening rate after β-arrestin-1 knockout were significantly lo...

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Abstract

The invention relates to the technical field of medicines, and provides a new application of a beta-arrestin-1 protein. The new application is the application of the beta-arrestin-1 in preparing a medicine or a health care product for preventing or treating myocardial infarction. Experiments prove that the myocardial infarction area of a mouse is outstandingly increased after the beta-arrestin-1 is knockout, the myocardial infarction area is outstandingly decreased after the beta-arrestin-1 is extremely expressed; in addition, left ventricular ejection fraction and left ventricular fractional shortening are obviously lower than those of a wild type mice group after the beta-arrestin-1 is knockout, and the left ventricular ejection fraction and the left ventricular fractional shortening are outstandingly enhanced after the beta-arrestin-1 is extremely expressed. Thus, the beta-arrestin-1 plays a protective role in the prognosis of the myocardial infarction.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of β-arrestin-1 in the preparation of drugs for preventing or treating myocardial infarction. Background technique [0002] β-arrestins (β-arrestins) are a group of multifunctional small molecular proteins, which play a very important role in regulating intracellular signal transduction. Its classic role is to participate in the desensitization and endocytosis of G protein-coupled receptors (reference 1. Shenoy SK, Lefkowitz RJ. β-Arrestin-mediated receptor trafficking and signal transduction. Trends Pharmacol Sci2011; 32:521-533.) . [0003] Recent studies have found that β-arrestins, especially β-arrestin-1 protein, as a "traffic member" of signal transduction, is also involved in many non-G protein-coupled receptor-activated signal transduction, in proteasome degradation, apoptosis And play an important role in a variety of cell signal transduction (referenc...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61K48/00A61P9/10
Inventor 刘冲邵柏棕苛苹徐哲琦苏定冯
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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