Magnolia liliiflora fermentation broth extract as well as preparation method and application thereof
A technology of purple magnolia and extract is applied in the field of tobacco flavors to achieve the effects of reducing cost, enhancing flavor and increasing smoke concentration
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Embodiment 1
[0028] 1. Media and culture conditions
[0029] The Saccharomyces longans CGMCC 6645 used in the experiment was inoculated in 50 mL of YPD liquid medium, and cultured at 28°C and 160 r / min for 24 hours to obtain activated liquid strains.
[0030] 2. Preparation of Magnolia samples
[0031] After the purple magnolia petals are fully dried, crush them and pass through a 40-mesh sieve. Weigh 20 g of magnolia petal powder, add 400 mL of distilled water (20 volume times), sterilize, and set aside.
[0032] 3. Aroma-producing yeast fermentation treatment of purple magnolia
[0033] Inoculate 8 mL of the activated Saccharomyces ulodesii liquid prepared in 1 into the magnolia sample prepared in 2 (the inoculum size is 2%), and culture it with shaking at 28°C and 160 r / min for 24 hours for the next experiment.
[0034] 4. Extraction of spice additives
[0035] After filtering the above fermented liquid, centrifuge at 8000r / min for 10 minutes, take the supernatant, concentrate it un...
Embodiment 2
[0048] Repeat Example 1, with the following differences:
[0049] The culture conditions for the activation of the strain CGMCC 6645 are 28°C, 160r / min shaking culture, and the culture time is 24 hours; the purple magnolia petals are crushed and 22 times distilled water is added to sterilize; the activated bacteria solution is inoculated on the purple magnolia material The inoculum amount is 2.5%; the fermentation and cultivation time after inoculation is 24 hours; the fermentation supernatant decompression concentration temperature is 55°C; the prepared spice additive is evenly sprayed on the shredded tobacco, and the addition amount is 12g / box.
[0050] The results of flavoring and smoking test show that the flavor additive can harmonize with the aroma of cigarettes, improve the quality of aroma, increase the amount of aroma, improve the aftertaste, cover up the miscellaneous smell, and have a fresh and special aroma.
Embodiment 3
[0052] Repeat Example 1, with the following differences:
[0053] The culture conditions for the activation of the strain CGMCC 6645 are 30°C, 180r / min shaking culture, and the culture time is 12 hours; the purple magnolia petals are crushed and 15 times distilled water is added to sterilize; the activated bacteria solution is inoculated on the purple magnolia material The inoculum amount is 1.5%; the fermentation and cultivation time after inoculation is 12 hours; the fermentation supernatant decompression concentration temperature is 45°C; the prepared spice additive is evenly sprayed on the shredded tobacco, and the addition amount is 8g / box.
[0054] The results of flavoring and smoking test show that the flavor additive can harmonize with the aroma of cigarettes, improve the quality of aroma, increase the amount of aroma, improve the aftertaste, cover up the miscellaneous smell, and have a fresh and special aroma.
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