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Magnolia liliiflora fermentation broth extract as well as preparation method and application thereof

A technology of purple magnolia and extract is applied in the field of tobacco flavors to achieve the effects of reducing cost, enhancing flavor and increasing smoke concentration

Inactive Publication Date: 2014-11-26
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the researches on magnolia are focused on the extraction of its volatile oil components. Using magnolia as a substrate and fermenting with aroma-producing yeast, the research on preparing tobacco flavor additives has not been reported yet.

Method used

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  • Magnolia liliiflora fermentation broth extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Media and culture conditions

[0029] The Saccharomyces longans CGMCC 6645 used in the experiment was inoculated in 50 mL of YPD liquid medium, and cultured at 28°C and 160 r / min for 24 hours to obtain activated liquid strains.

[0030] 2. Preparation of Magnolia samples

[0031] After the purple magnolia petals are fully dried, crush them and pass through a 40-mesh sieve. Weigh 20 g of magnolia petal powder, add 400 mL of distilled water (20 volume times), sterilize, and set aside.

[0032] 3. Aroma-producing yeast fermentation treatment of purple magnolia

[0033] Inoculate 8 mL of the activated Saccharomyces ulodesii liquid prepared in 1 into the magnolia sample prepared in 2 (the inoculum size is 2%), and culture it with shaking at 28°C and 160 r / min for 24 hours for the next experiment.

[0034] 4. Extraction of spice additives

[0035] After filtering the above fermented liquid, centrifuge at 8000r / min for 10 minutes, take the supernatant, concentrate it un...

Embodiment 2

[0048] Repeat Example 1, with the following differences:

[0049] The culture conditions for the activation of the strain CGMCC 6645 are 28°C, 160r / min shaking culture, and the culture time is 24 hours; the purple magnolia petals are crushed and 22 times distilled water is added to sterilize; the activated bacteria solution is inoculated on the purple magnolia material The inoculum amount is 2.5%; the fermentation and cultivation time after inoculation is 24 hours; the fermentation supernatant decompression concentration temperature is 55°C; the prepared spice additive is evenly sprayed on the shredded tobacco, and the addition amount is 12g / box.

[0050] The results of flavoring and smoking test show that the flavor additive can harmonize with the aroma of cigarettes, improve the quality of aroma, increase the amount of aroma, improve the aftertaste, cover up the miscellaneous smell, and have a fresh and special aroma.

Embodiment 3

[0052] Repeat Example 1, with the following differences:

[0053] The culture conditions for the activation of the strain CGMCC 6645 are 30°C, 180r / min shaking culture, and the culture time is 12 hours; the purple magnolia petals are crushed and 15 times distilled water is added to sterilize; the activated bacteria solution is inoculated on the purple magnolia material The inoculum amount is 1.5%; the fermentation and cultivation time after inoculation is 12 hours; the fermentation supernatant decompression concentration temperature is 45°C; the prepared spice additive is evenly sprayed on the shredded tobacco, and the addition amount is 8g / box.

[0054] The results of flavoring and smoking test show that the flavor additive can harmonize with the aroma of cigarettes, improve the quality of aroma, increase the amount of aroma, improve the aftertaste, cover up the miscellaneous smell, and have a fresh and special aroma.

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Abstract

The invention discloses a magnolia liliiflora fermentation broth extract as well as a preparation method and application thereof. The extract is prepared by fermenting a raw material magnolia liliiflora with lodderomyces elongisporus and then extracting aroma components from fermentation broth. Lodderomyces elongisporus is collected in the China General Microbiological Culture Collection Center (CGMCC), with collection number of CGMCC No.6645 and collection date of October 08, 2012. The product contains various aroma components, such as phenethyl alcohol, myrcene, caryophyllene, linalool, megastigmatrienone and the like. Flavoring smoke panel test results show that the extract can harmonize with cigarette aromas and can obviously improve and regulate cigarette aromas, refine alcohols and smoke, reduce the irritation, hide offensive odors, improve the smoking flavors of cigarettes and produce fresh special aroma.

Description

technical field [0001] The invention belongs to the technical field of tobacco additives, and in particular relates to a tobacco flavor obtained by extracting aroma components from fermented magnolia. At the same time, the present invention also relates to the preparation method of the tobacco spice and its specific application in tobacco processing. Background technique [0002] Cigarette aroma is the core content of cigarette quality. Whether it is international or domestic, reducing the amount of tar has become an important issue in cigarette production, and with the reduction of the amount of tar, the taste of cigarettes becomes lighter and the aroma is weakened, which is difficult to be accepted by consumers. While reducing cigarette tar, it is an effective compensation method to increase and improve smoke concentration and flavor by adding spice additives. [0003] Natural flavors and fragrances are high-value fine chemical products, but the original natural flavors ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02A24B3/12C12R1/645
Inventor 党立志王坚段焰青陈兴朱保昆王文元范多青刘亚
Owner CHINA TOBACCO YUNNAN IND
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