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Application of water-soluble silicon quantum dots in the detection of dopamine

A technology of silicon quantum dots and water-soluble silicon, which is applied in the field of analysis and detection, can solve the problems of poor detection specificity, cumbersome preparation methods, and low detection sensitivity, and achieve the effects of good specificity, simple synthesis method, and high detection sensitivity

Inactive Publication Date: 2016-11-02
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still many deficiencies in the prior art using fluorescent methods to detect dopamine, such as low detection sensitivity, poor detection specificity (susceptible to interference from other components), complex detection methods (complex preparation methods for fluorescent probes), high detection cost, etc.

Method used

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  • Application of water-soluble silicon quantum dots in the detection of dopamine
  • Application of water-soluble silicon quantum dots in the detection of dopamine
  • Application of water-soluble silicon quantum dots in the detection of dopamine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for preparing water-soluble high-brightness fluorescent silicon quantum dots, comprising the following steps:

[0033] (1) feed nitrogen into the sodium citrate solution for 5 minutes to remove oxygen in the solution;

[0034] (2) Add the silane reagent aminopropyltrimethoxysilane (APTMS) while stirring, and stir for 10min in a closed state to form a silicon quantum dot precursor solution;

[0035] (3) React in a microwave reactor at 140° C. for 15 minutes to form a silicon quantum dot solution;

[0036] (4) Dialyzing the silicon quantum dot solution with a dialysis bag with a molecular weight of 1000 to obtain a pure silicon quantum dot solution.

[0037] The transmission electron microscopy results of the figure 1 , the UV-Vis absorption spectrum results are shown in figure 2 , fluorescence excitation at 444nm and emission spectrum at 348nm see image 3 .

Embodiment 2

[0039] A method for preparing water-soluble high-brightness fluorescent silicon quantum dots, comprising the following steps:

[0040] (1) feed nitrogen into the sodium citrate solution for 10 minutes to remove oxygen in the solution;

[0041] (2) Add the silane reagent aminopropyltriethoxysilane (APTES) while stirring, and stir for 5 minutes in a closed state to form a silicon quantum dot precursor solution;

[0042] (3) React in a microwave reactor at 180° C. for 5 minutes to form a silicon quantum dot solution;

[0043] (4) Dialyzing the silicon quantum dot solution with a dialysis bag with a molecular weight of 1000 to obtain a pure silicon quantum dot solution.

[0044] Its transmission electron microscope pattern, ultraviolet-visible absorption spectrum, fluorescence excitation at 444nm and emission spectrum at 348nm are consistent with Example 1.

Embodiment 3

[0046] Silicon quantum dots with a concentration of 1mg / mL and 20μM dopamine are mixed in equal volumes, and under the condition of standing at room temperature (25°C), the curve of the fluorescence intensity of silicon quantum dots changing with time is measured, and the results are shown in Figure 4 .

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Abstract

The invention provides application of a water-soluble silicon quantum dot to dopamine detection. According to a theory that the fluorescence of a silicon quantum dot is quenched by virtue of electron transferring between dopamine and the silicon quantum dot, the concentration of dopamine in a sample is detected. The application has the advantages of simplicity in sample synthesis, high sensitivity, wide detection range, high specificity, low detection cost and the like, also has the advantages of small silicon quantum dot size, low toxicity and high photobleaching-resistant performance, and can be a method for monitoring dopamine in vivo or in vitro for a long time.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to the application of water-soluble silicon quantum dots in the detection of bardomamine. Background technique [0002] Dopamine is the most important catechol neurotransmitter in the central nervous system of the human brain, and it is involved in many brain functions and behavioral responses. Excessive dopamine secretion can cause excessive energy and energy consumption, leading to premature death, such as Huntington's disease; insufficient dopamine secretion can cause people to lose the ability to control muscles, or cause inability to concentrate, and even Parkinson's disease in severe cases . [0003] The detection methods of dopamine in the prior art mainly include chromatography, electrochemical method, fluorescence method and capillary electrophoresis method and the like. Fluorescence is widely used because of its high sensitivity. However, ther...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 吴富根张晓东陈晓凯
Owner SOUTHEAST UNIV