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Construction of a mouse model of pulmonary Candida albicans infection after transplantation

A technology of Candida albicans and mouse models, applied in plant/algae/fungi/moss components, pharmaceutical formulas, plant raw materials, etc., can solve the problems that are not close to the actual clinical infection situation and do not consider transplantation factors, etc. To achieve the effect of model stability

Active Publication Date: 2017-05-17
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is to overcome the defect that the mouse model of Candida albicans infection in the prior art is not too close to the actual clinical infection situation, and does not consider transplantation factors, etc., to provide a kind of candida albicans after transplantation. Construction method of yeast infection mouse model

Method used

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  • Construction of a mouse model of pulmonary Candida albicans infection after transplantation
  • Construction of a mouse model of pulmonary Candida albicans infection after transplantation
  • Construction of a mouse model of pulmonary Candida albicans infection after transplantation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] 1. Establishment of mouse low back skin graft model

[0057] The following operations need to be carried out in an SPF-level environment. The relevant experimental supplies must be treated with corresponding procedures (high temperature and high pressure, spraying 75% alcohol and ultraviolet radiation) and then enter the barrier environment. The experimental animals must be inspected and quarantined in advance, and can only be used after they adapt to the experimental environment. use.

[0058] 1. Item preparation: ophthalmic scissors, toothless tweezers, curved tweezers, vernier calipers, marker pens, needle holders, glass plates, 10ml syringes, 1ml syringes, shaver, small horn needles, silk thread, Vaseline gauze, sterile gauze, Medical bandages, medical tape, electronic balance, 15ml sterile centrifuge tube.

[0059] 2. Drug preparation: anesthetics, 75% medical alcohol, tincture of iodine, sterile saline, cyclosporine A.

[0060] 3. Selection of mice: male C57BL / 6...

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Abstract

The invention relates to the technical field of preparation of animal models, and specifically discloses a construction method of a post-transplantant candida albicans pulmonary infection mouse model. The construction method comprises the following steps: selecting a male C57BL / 6J mouse at the age of six to eight weeks as a donator, a male Balb / c mouse at the age of six to eight weeks as a receptor, and ciclosporin A as an immunosuppressor to construct a mouse skin transplantation model; and dropping 200 mu l of 5*10<5> CFU / ml candida albicans suspension into the body of the mouse suffering from lower back skin transplantation operation 7 days after the skin transplantation operation to obtain the post-transplantant candida albicans pulmonary infection mouse model. According to the construction method of the post-transplantant candida albicans pulmonary infection mouse model, the prepared mouse model is closer to the actual occurrence condition of post-transplantant fungal infection of a clinical patient; in addition, the model is stable; moreover, the method is simple and fast; a large number of stable mouse infection models can be obtained at a time for researchers to use in relevant study on clinical problems.

Description

technical field [0001] The invention relates to the technical field of animal model preparation, and specifically discloses a method for constructing a mouse model of Candida albicans infection after transplantation. Background technique [0002] Infection, rejection, and cardiovascular and cerebrovascular diseases are currently the main reasons affecting the prognosis of transplant recipients. Due to factors such as long operation time, heavy intraoperative bleeding, and postoperative administration of immunosuppressants for organ transplant patients, postoperative patients have low immunity and a high incidence of infection, which seriously affects the long-term survival rate and quality of life of patients. [0003] The immune system of transplant recipients is significantly different from that of the general population, and there are large differences in infection spectrum, infection characteristics, clinical manifestations, diagnosis and treatment. These factors greatly...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/064A61D1/00
Inventor 何晓顺郭志勇张钰杨安力吴玉娥黄韧
Owner THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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