Method for extracting arabinoxylan from Astragalus medicinal residues by using ionic liquid
A technology of arabinoxylan and ionic liquid, which is applied in the field of traditional Chinese medicine extraction, can solve problems such as the difficulty of separating and purifying arabinoxylan, achieve good social and economic benefits, avoid environmental pollution, and avoid low yields of enzymatic methods Effect
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Embodiment 1
[0019] 1), extraction of arabinoxylan: After the dried astragalus medicinal residues are pulverized, pass through a 100-mesh sieve for subsequent use; add the above-mentioned powder to porcine pancreatic α-amylase (Tris-HCl buffer solution, pH7.0, 200U / mL ) solution (mass ratio of powder to porcine pancreatic α-amylase solution: 1:10), shake overnight at 37°C. After removing the starch, centrifuge at 5000 rpm and discard the supernatant. The precipitate was washed with deionized water, centrifuged at 5000 rpm, and the supernatant was discarded to collect the precipitate. After repeated washing three times, dry it for later use. Add 5g of ionic liquid [Bmim]NO to 1g of the above-mentioned medicinal dregs powder after starch removal 3 (1-butyl-3-methylimidazole nitrate), magnetically stirred at 65°C for 36 hours. After stirring and cooling, add 200 mL of deionized water, let it stand and remove the precipitate, and recover the filtrate. Concentrate the filtrate to remove wat...
Embodiment 2
[0027] After the dried Radix Astragali dregs are crushed, pass through a 100-mesh sieve and set aside; add the above powder into porcine pancreatic α-amylase (Tris-HCl buffer, PH7.0, 200U / mL) solution (powder and porcine pancreatic α-amylase Enzyme solution mass ratio 1:20), shake overnight at 37°C. After removing the starch, centrifuge at 5000 rpm and discard the supernatant. The precipitate was washed with deionized water, centrifuged at 5000 rpm, and the supernatant was discarded to collect the precipitate. After repeated washing three times, dry it for later use. Add 50g of ionic liquid [Bmim]NO to 5g of the above-mentioned dregs powder after starch removal 3 (1-butyl-3-methylimidazole nitrate), magnetically stirred at 90°C for 18 hours. After stirring and cooling, add 600 mL of deionized water, let it stand and remove the precipitate, and recover the filtrate. Concentrate the filtrate to remove water, add 200mL tetrahydrofuran, and let it stand to collect the precipit...
Embodiment 3
[0029] After the dried Radix Astragali dregs were crushed, passed through a 100-mesh sieve, and set aside; the above powder was added to porcine pancreatic α-amylase (Tris-HCl buffer, PH7.0, 200U / mL) solution (powder and porcine pancreatic α-amylase Enzyme solution mass ratio 1:15), shake overnight at 37°C. After removing the starch, centrifuge at 5000 rpm and discard the supernatant. The precipitate was washed with deionized water, centrifuged at 5000 rpm, and the supernatant was discarded to collect the precipitate. After repeated washing three times, dry it for later use. Add 40g of ionic liquid [Bmim]NO to 3g of the above-mentioned medicinal dregs powder after starch removal 3 (1-butyl-3-methylimidazole nitrate), magnetically stirred at 70°C for 36 hours. After stirring and cooling, add 400 mL of deionized water, let it stand and remove the precipitate, and recover the filtrate. Concentrate the filtrate to remove water, add 150 mL of tetrahydrofuran, and let it stand t...
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