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Content detection method of traditional Chinese medicine compound granules for treatment of gynecological anemia

A detection method and blood disease technology, which is applied in the direction of measuring devices, scientific instruments, instruments, etc., can solve the problems of large negative interference, inability to effectively control the content of protocatechuic acid, and inaccurate determination, so as to eliminate negative interference and ensure production The effect of controllable process and simple operation

Active Publication Date: 2016-08-31
广西万寿堂药业有限公司
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

This method has a large negative interference on the main active ingredient protocatechuic acid, the determination is not accurate, and the content of protocatechuic acid in the finished product cannot be effectively controlled.

Method used

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  • Content detection method of traditional Chinese medicine compound granules for treatment of gynecological anemia
  • Content detection method of traditional Chinese medicine compound granules for treatment of gynecological anemia
  • Content detection method of traditional Chinese medicine compound granules for treatment of gynecological anemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation of embodiment 1 need testing solution

[0025] Take the traditional Chinese medicine compound granules, grind them finely, take about 4g, accurately weigh, add 50ml of methanol, heat and reflux for 1 hour, filter, evaporate the filtrate to dryness, add 20ml of 1mol / L hydrochloric acid to the residue, filter, extract the filtrate with ether three times, 15ml each time, combine the ether solution, put it in a conical flask containing 4g of anhydrous sodium sulfate and let it stand for 30min, divide the ether solution and evaporate to dryness, dissolve the residue with an appropriate amount of methanol, transfer it to a 10ml measuring bottle, and add methanol to the mark , Shake well, and filter through a 0.45 μm microporous membrane.

Embodiment 2

[0026] Example 2 Screening the detection conditions of the content of protocatechuic acid and protocatechuic aldehyde in Chinese medicine compound granules

[0027] Screening of chromatographic conditions: Methanol-glacial acetic acid: water (10:90:1), methanol-1% glacial acetic acid (90:10), methanol-water (20:80, adjust pH=2.8 with glacial acetic acid), Acetonitrile-1% glacial acetic acid and methanol (A), 1% glacial acetic acid (B) gradient elution and other mobile phase systems, after experiments and comparisons, it is finally determined that methanol (A), 1% glacial acetic acid (B) gradient elution completes the above-mentioned Determination of the content of the two components.

[0028] The effects of different column temperatures of 25°C, 30°C, and 40°C; and different flow rates of 0.8, 1.0, and 1.2mL / min on the separation of active components were investigated. The test results showed that the separation effect was the best at 25°C and flow rate of 1.0mL / min.

[0029]...

Embodiment 3

[0032] Content detection of protocatechuic acid and protocatechuic aldehyde in Chinese medicine compound granule (Yixue'an granule) in embodiment 3

[0033] Chromatographic conditions and system suitability test

[0034] Octadecyl silanized bonded silica gel is used as a filler; the mobile phase is composed of methanol and 1% glacial acetic acid, and gradient elution is carried out. In 0-6min, the volume fraction of methanol is 10%, and in 6-10min, the volume fraction of methanol is From 10% to 22%, 10-20min, the methanol volume fraction maintains 22%, 20-25min, the methanol volume fraction changes from 22% to 55%, 25-40min, the methanol volume fraction maintains 55%; the detection wavelength is 280nm, Flow rate 1.0ml / min, column temperature 25°C, injection volume: 10μl The number of theoretical plates is not less than 3000 based on protocatechuic acid and protocatechuic aldehyde peaks;

[0035] Preparation of reference solution

[0036] Precisely weigh 6.26mg of protocatech...

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Abstract

The invention discloses a method for detecting the content of traditional Chinese medicine compound granules for treating gynecological diseases. The content of protocatechuic acid and protocatechuic aldehyde is simultaneously measured under the same chromatographic condition. Acidic conditions obtained by extraction with ether. This effectively eliminates the negative interference, and can increase the extraction rate of protocatechuic acid and protocatechuic aldehyde. The method of the present invention has good stability, good reproducibility, strong specificity and simple operation. Based on this, a new quality control standard for Yixuan granules can be established, which can help effectively control the quality of finished products in the production of Yixuan granules and improve their quality. The quality level ensures the controllable production process, ensures the curative effect of clinical use, and provides a reliable guarantee for the stable and accurate curative effect of the product.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine content detection, and in particular relates to a content detection method of traditional Chinese medicine compound granules used for treating gynecological anemia. Background technique [0002] Yixuean granules and Yixuean (sugar-free) granules are traditional Chinese medicine compound granules used to treat gynecological anemia. They are composed of Diangui Ainaxiang, Motherwort, Corydalis Corydalis, Licorice and other herbs. They have the functions of promoting blood circulation and stopping bleeding, promoting Qi and relieving pain . It is used for persistent lochia after childbirth, unclean uterine bleeding after induced abortion, and blood stasis syndrome according to TCM syndrome differentiation. It can shorten the duration of bleeding and relieve abdominal pain. In the standard YBZ00292008 of the State Food and Drug Administration, the content determination method of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/34
Inventor 蒋冰清罗盛东李修善
Owner 广西万寿堂药业有限公司
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