Haemophilus parasuis test paper strip and preparation method thereof

Abstract

The invention discloses a haemophilus parasuis type 5 colloidal gold formed kit and a preparation method thereof. The test paper strip comprises a sample pad, a colloidal gold pad, a nitrocellulose membrane and an absorbent pad which are orderly connected and fixed on a PVC (polyvinyl chloride) plate, and also comprises a detection line and a quality control line, the sample pad comprises a water absorbent material, the colloidal gold pad is coated with antigen, the detection line and the quality control line are located on the nitrocellulose membrane and are separated from each other, the antigen coating the colloidal gold pad is haemophilus parasuis OPPA (oligopeptide binding protein A) fusion protein, the detection line is haemophilus parasuis type 5 antigen, and the quality control line is haemophilus parasuis whole bacterial antigen polyclonal antibody IgG. The test paper strip has the advantages of good specificity, strong sensitivity and repeatability.

Description

technical field [0001] The invention relates to a disease detection reagent used in animal husbandry and a preparation method thereof, in particular to a Haemophilus parasuis type 5 colloidal gold typing kit and a preparation method of the kit. Background technique [0002] Due to the large number of serotypes of Haemophilus parasuis, 15 serotypes are currently known, and there are many methods for detecting the pathogen and antibodies, but they are all species-specific. The detection of Haemophilus parasuis disease Methods include PCR method for detecting antigens, indirect hemagglutination method and ELISA method for detecting serum antibodies. Only the agar amplification test developed by the applicant can be used for stereotype detection. The preparation of agar expansion test is complicated, and it takes 2-3 days for the detection to produce results. In addition, the serum to be tested needs to be concentrated, and the requirements for antigens are high. Haemophilus pa...

AI Technical Summary

Problems solved by technology

[0002] Due to the large number of serotypes of Haemophilus parasuis, 15 serotypes are currently known, and there are many methods for detecting the pathogen and antibodies, but they are all species-specific. The detection of Haemophilus parasuis disease Methods include PCR method for detecting antigens, indirect hemagglutination method and ELISA method for detecting serum antibodies. Only the agar amplification test developed by the applicant can be used for stereotype detection. The preparation of agar expansion test is complicated, and it takes 2-3 days for the detection to produce results. In addition, the tested serum needs to be concentrated, and the requirements for antigens are high

Haemophilus parasuis type 5 is one of the popular strains of Haemophilus parasuis disease in my country at present, but the existing technology lacks antibodies capable of typing and detecting type 5 serotypes, especially rapid detection methods, thus affecting field epidemics Scientific investigation and accurate prevention and control work

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