Rapid propagation method for tissue culture of momordica cochinchinensis (Lour.) Spreng

A technique for tissue culture and Momordica charcoal is applied in the field of plant cultivation and can solve the problems of low reproduction rate, long growth cycle and high cost

Inactive Publication Date: 2015-01-07
NANJING DIDAO AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mainly adopt the seed and root head propagation method, the reprod

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Select smooth and plump seeds, soak them in concentrated sulfuric acid for 1 hour, rinse them repeatedly with tap water and sow them in flower pots with fine sand as the substrate, place the flower pots in a place with sufficient light, and place the leaves of the cultivated wood beetle seedlings on the ground with clean gauze. Wash in water with detergent, soak in 10% hydrogen peroxide for 6 minutes, rinse with sterile water 5 times, cut into small pieces of 0.5cm×0.5cm and put them into MS+IBA0.02mg / L+ZT3mg / L medium , add 0.7% agar, 35g / L sucrose to induce callus, pH5.85, culture in darkroom, temperature 27℃, put the induced callus into ER+IAA0.1mg / L+5mg / L2ip+PVP0. Carry out callus differentiation in 2g / L+0.7% agar+35g / L sucrose medium, pH 5.85, light 3000lx, temperature 27°C, take the sprouts out of the culture bottle and put them in 0.3% sodium sulfate solution Soak for 3 minutes, rinse with sterile water, put it into the sterilized perlite substrate, pour MS nutrie...

Embodiment 2

[0011] Select smooth and plump seeds, soak them in concentrated sulfuric acid for 1 hour, rinse them repeatedly with tap water and sow them in flower pots with fine sand as the substrate, place the flower pots in a place with sufficient light, and place the leaves of the cultivated wood beetle seedlings on the ground with clean gauze. Wash in water with detergent, soak in 10% hydrogen peroxide for 6 minutes, rinse with sterile water 5 times, cut into small pieces of 0.5cm×0.5cm and put them into MS+IBA0.02mg / L+ZT3mg / L medium , add 0.7% agar, 35g / L sucrose to induce callus, pH5.85, culture in darkroom, temperature 27°C, put the induced callus into ER+IAA0.15mg / L+6mg / L2ip+PVP0. Carry out callus differentiation in 25g / L+0.7% agar+35g / L sucrose medium, pH 5.85, light 3000lx, temperature 27°C, take the sprouts out of the culture bottle and put them in 0.3% sodium sulfate solution Soak for 3 minutes, rinse with sterile water, put it into the sterilized perlite substrate, pour MS nut...

Embodiment 3

[0013] Select smooth and plump seeds, soak them in concentrated sulfuric acid for 1 hour, rinse them repeatedly with tap water and sow them in flower pots with fine sand as the substrate, place the flower pots in a place with sufficient light, and place the leaves of the cultivated wood beetle seedlings on the ground with clean gauze. Wash in water with detergent, soak in 10% hydrogen peroxide for 6 minutes, rinse with sterile water 5 times, cut into small pieces of 0.5cm×0.5cm and put them into MS+IBA0.02mg / L+ZT3mg / L medium , add 0.7% agar, 35g / L sucrose to induce callus, pH5.85, culture in darkroom, temperature 27℃, put the induced callus into ER+IAA0.1mg / L+6mg / L2ip+PVP0. Carry out callus differentiation in 2g / L+0.7% agar+35g / L sucrose medium, pH 5.85, light 3000lx, temperature 27°C, take the sprouts out of the culture bottle and put them in 0.3% sodium sulfate solution Soak for 3 minutes, rinse with sterile water, put it into the sterilized perlite substrate, pour MS nutrie...

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PUM

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Abstract

The invention discloses a rapid propagation method for tissue culture of momordica cochinchinensis (Lour.) Spreng. The method comprises steps of obtaining of seedlings of momordica cochinchinensis (Lour.) Spreng, induction of callus tissue, differentiation of callus tissue, rooting induction and the like. According to the plant tissue culture technology, a method for establishing an asexual propagation system for momordica cochinchinensis (Lour.) Spreng is researched with a tissue culture method, and a technological base is provided for fine variety selection and rapid propagation of momordica cochinchinensis (Lour.) Spreng.

Description

technical field [0001] The invention relates to a rapid propagation method for tissue culture of jacobs, and belongs to the technical field of plant cultivation. Background technique [0002] wood turtle, Momordica cochinchinensis (Lour.) Spreng. , Cucurbitaceae, another name: Snakewood turtle, glutinous rice fruit, mouse pull wax gourd and so on. Perennial herbaceous rattan, likes warm and humid climate and sunny environment. The soil conditions are not strict, and it is advisable to choose well-drained, fertile and deep sandy loam for cultivation. It often grows in places with deep soil layers such as ravines, forest edges, and roadsides at an altitude of 450-1100 meters. Distributed in Jiangsu, Anhui, Jiangxi, Fujian, Taiwan, Guangdong, Guangxi, Hunan, Sichuan, Guizhou, Yunnan and Tibet. Indochina peninsula and Indian peninsula also have. Contains gaconic acid, carnation, oleanolic acid, α-cartonic acid, amino acids, sterols. Dispersing stagnation and detumescence, a...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 刘东锋杨成东
Owner NANJING DIDAO AGRI SCI & TECH
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