hcmv infection diagnostic reagent

Abstract

The invention provides a diagnostic reagent for human cytomegalovirus infection. The diagnostic reagent comprises human cytomegalovirus DNA PCR amplification primers, a miRNA-UL112-3p forward PCR amplification primer, and a miRNA-UL112-5p forward PCR amplification primer. The diagnostic reagent adopts a PCR method, and is used for detecting the miR-UL112 level of a peripheral blood mononuclear cell of non-tumorous patients (comprising 57 patients with positive HCMV-specific CD8<+>T lymphocytes and 35 patients with negative HCMV-specific CD8<+>T lymphocytes) and tumor patients treated with chemotherapy; the detection results are that levels of miR-UL-112-3p and 5p among groups are significantly different; and the detection results show that the levels of the miR-UL-112-3p and the 5p have certain diagnostic value for HCMV latency and excitation of infection.

Description

technical field [0001] The invention relates to a reagent for diagnosing HCMV infection, in particular to a reagent for diagnosing HCMV infection according to the level of miRNA-UL112 in human peripheral blood mononuclear cells. Background technique [0002] Human cytomegalovirus (humancytomegalovirus, HCMV) belongs to the herpes virus family, is the largest virus in the human herpesvirus group, and can cause human cytomegalovirus infection. HCMV is generally susceptible in the population, and the infection rate of HCMV in adults is as high as 90%. Usually, there are no obvious symptoms, and it is latent in the host cells, which is a recessive infection. When the immune function is low or defective, such as pregnancy, multiple blood transfusions , transplant recipients (HCMV virus infection is also one of the main reasons for organ transplant failure), AIDS patients, etc., often present with provocative infection (dominant infection), the virus can invade multiple organs and...

AI Technical Summary

Problems solved by technology

[0014] Judging from the current PCR detection results, a single pair of PCR primers can cause false negatives, which may be caused by differences in HCMV strains in clinical specimens, minimal variation in the virus genome, and too low a copy number of the virus

In addition, the primer design sequence is aimed at different parts of the same HCMV gene, and the PCR results are also significantly different. The method needs to be standardized. There is no commercial HCMV PCR diagnostic kit on the market.

[0015] In summary, there is currently no convenient and clear method to clearly distinguish between latent and provocative infection of HCMV.

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