Plant organic matter-bacillus subtils mixed inoculum and its preparation method and use

A technology of Bacillus subtilis and plant organic matter, applied in the directions of botanical equipment and methods, biochemical equipment and methods, plant growth regulators, etc., can solve problems such as no relevant reports, achieve broad market prospects, good control effects, Good control effect

Active Publication Date: 2015-01-14
深圳华农中绿生物科技研究有限公司
View PDF12 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Based on the above ideas and technical requirements, the present invention adopts an adsorption carrier and a production process that are different from traditional biological agents, and uses plant organic matter such as neem residue, tea dead, and palm kernel meal as the raw material of the culture medium for biocontrol bacteria and the production process

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Plant organic matter-bacillus subtils mixed inoculum and its preparation method and use
  • Plant organic matter-bacillus subtils mixed inoculum and its preparation method and use
  • Plant organic matter-bacillus subtils mixed inoculum and its preparation method and use

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0046] Example 1 Enrichment screening and identification of Bacillus subtilis HN09

[0047] 1. Enrichment screening of Bacillus subtilis HN09

[0048] The soil samples collected in the Insecticidal Plant Herbarium of South China Agricultural University in Guangzhou City, Guangdong Province were used as soil samples. Weigh 10 g of soil into a triangular flask containing sterilized plant organic medium (30 g of neem residue, 30 g of tea leaves, 30 g of palm kernel meal, 5 g of peptone, 3 g of yeast powder, natural pH) for enrichment and cultivation for one week. Then, weigh 10 g of the enriched culture and put it into a Erlenmeyer flask containing 90 mL of sterile water, shake it well and let it stand for 10 min. Take 1mL of supernatant and dilute to 10 with a 10-fold gradient -4 , 10 -5 , 10 -6 Concentration, respectively take 0.1mL dilution, spread it on LB medium (10g peptone, 5g yeast powder, 10g NaCl, 15g agar powder, 1L distilled water, natural pH, sterilize at 121℃ for 30min)...

Example Embodiment

[0054] Example 2 Experiment of Bacillus subtilis HN09 producing volatile gas with antibacterial activity

[0055] The activated Bacillus subtilis HN09 was inoculated into a nutrient broth medium and cultured on a shaker at 180 rpm / min at 30°C for 24 hours. Take 200μ1 strain HN09 and evenly spread it on the pre-prepared gas production medium (10g peptone, 5g yeast powder, 3g beef extract, 15g glucose, K 2 HPO 4 2g, FeSO 4 0.2g, 20g agar, 1L distilled water, pH 6.8~7.2) on the plate; insert a 5mm diameter FOC4 bacterial cake in the center of another plate of PDA medium, and then invert it on the gas production medium plate containing strain HN09 ( Treatment group), with the plate without bacterial liquid as the control, the gap between the upper and lower plates was sealed with a sealing film, and the plates were incubated at 28°C in the dark. Set 3 repetitions. After the control FOC4 hypha grows to 1 cm from the edge of the plate, measure the growth distance of the hypha and calc...

Example Embodiment

[0056] Example 3 Preparation of plant organic inoculum of Bacillus subtilis HN09

[0057] (1) Preparation of first-level seed liquid

[0058] The seed liquid medium is a nutrient broth liquid medium: beef extract 0.5%, peptone 0.5%, NaCl 0.5%, the rest is distilled water, pH 6.5-7.3. Sterilize the medium at 121°C for 20 minutes, insert the activated Bacillus HN09, and culture it in a shake flask. Culture conditions: rotation speed 180rpm, 30°C, culture time 24h.

[0059] (2) Preparation of secondary seed liquid

[0060] The formula of the secondary seed culture medium is 1-3% corn flour, 0.5-2% soybean flour, 0.1-0.3% yeast powder, K 2 HPO 4 0.05~0.15%, MgSO 4 0.01~0.03%, CaCO 4 0.1-0.2%, the rest is water, pH 6.5-7.3. The culture medium is sterilized at 121°C for 20 minutes, and when the temperature drops to about 30°C, the first-level seed solution is inserted into the seed tank at a volume ratio of 5 to 15% for cultivation. The ventilation rate is 1:1, the stirring speed is 100-...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a plant organic matter-bacillus subtils mixed inoculum and its preparation method and use. The preparation method comprises that one or more of plant organic matters of azadirachta residue, tea cake and palm kernel meal as bacillus subtils HN09 medium main raw materials and an inoculums adsorption carrier are subjected to two-step fermentation decomposition so that the plant organic matter-bacillus subtils mixed inoculum is obtained. Active components of the plant organic matter-bacillus subtils mixed inoculum comprise the plant organic matters, bacillus subtils HN09 and fermentation metabolite of the bacillus subtils HN09. The bacillus subtils HN09 is preserved in the China general microbiological culture collection center (CGMCC) on April 26, 2013 and has a preservation number of CGMCC NO. 7524. The bacillus subtils HN09 and the plant organic matter-bacillus subtils mixed inoculum can be used for preventing and controlling plant diseases and soil insects, can promote plant growth and nitrogen absorption of plants, have important meaning for enlarging an insect disease prevention and control scope and promoting agricultural waste recycle and have a wide application prospect in production practice.

Description

technical field [0001] The invention belongs to the field of biological pesticides and plant protection, and in particular relates to a preparation method of bacillus subtilis strain HN09 and its plant organic matter-biocontrol bacteria mixed bacterial agent, and its application in plant disease and insect pest control. Background technique [0002] Neem slag, tea seed and palm kernel meal are the leftovers of agricultural by-products in the processing of neem seeds, tea seeds and palm kernels. Due to the low utilization rate, they are usually discarded as waste. This is obviously not in line with the purpose of sustainable agricultural development, so it is necessary to find a way to effectively reuse these agricultural by-product waste. In view of the fact that these plant organic matter still contains a large amount of organic matter and plant protein and other nutrients, it can be used as a medium material for microorganisms. Adding biocontrol bacteria to these plant org...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01N63/02A01P3/00A01P7/04A01P21/00C12N1/20C12R1/125
Inventor 徐汉虹赖多康向辉
Owner 深圳华农中绿生物科技研究有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap