Method for breeding ecotype recessive nuclear male sterile two-purpose line of oilseed rape
A male sterile line and male sterility technology, which is applied in the field of agricultural biology, can solve the problems of the complex technology of the whole sterile line and the problem of the stability of ecotype nuclear male sterility, and achieves obvious selection markers and controllable problems. Convenient, Breeding Simple Effects
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Embodiment 1
[0018] Example 1: Breeding and propagation of ERGMS
[0019] a. select the low-temperature recessive nuclear male sterile line (LTRGMS) and the cytoplasmic male sterile line (CMS) of the Porrima type to cross to obtain the first generation of hybrids (F1);
[0020] b. sow the F1 generation seeds, identify the fertility of the F1 generation, and select fertile plants for selfing to obtain the F2 generation;
[0021] c. Identify the fertility of the F2 generation plants under autumn sowing conditions, and select the low-temperature fertile and high-temperature sterile plants for selfing or artificially assisted pollination selfing to obtain the F3 generation;
[0022] d. The seeds of the F3 generation are divided into two parts, one part is reserved, and the other part is sown on a single plant under the conditions of spring sowing or summer sowing, and the fertility is identified at the flowering stage;
[0023] e. Sow the reserved seeds of the sterile strains under spring or ...
Embodiment 2
[0028] Example 2: ERGMS hybrid production
[0029] Under the conditions of spring sowing or summer sowing, in the natural barrier isolation area, the ERGMS dual-purpose line and the restorer line were planted alternately at a row ratio of 3:1 or 4:1 or 5:2 or 6:2. Hybrid F1 seeds were harvested on the ERGMS dual-purpose line when the seeds were mature.
Embodiment 3
[0030] Embodiment 3: Breeding practice
[0031] In March 2004, the low-temperature sterile recessive nuclear male sterile line 950 of Brassica napus was crossed with the low-temperature fertile high-temperature sterile cytoplasmic male sterile line Pol CMS 1141A of Brassica napus to obtain F1 generation seeds.
[0032] In May 2004, under the condition of summer sowing in Changyang County, Hubei Province, the F1 generation seeds were sown. The F1 generation plants were fertile, and they were self-crossed to obtain the F2 generation seeds.
[0033] In October 2004, seeds of the F2 generation were sown under autumn sowing conditions in Wuhan City. In the spring of 2005, the males of the F2 generation plants separated from fertile and sterile, and the plants that were fertile at low temperature and sterile at high temperature were selected for self-bagging, and obtained F3 selfing seeds.
[0034] In May 2005, the low-temperature fertile and high-temperature sterile F3 self-bred s...
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