Tissue culture rapid propagation method of acer palmatum

A technique for tissue culture and maple japonica is applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of difficult rooting, low survival rate of cuttings, etc., and achieves low cost, increased propagation base, and simple formula. Effect

Inactive Publication Date: 2015-01-21
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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Problems solved by technology

Acer palmatum is a tree species that is difficult to root, and the survival rate o...

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  • Tissue culture rapid propagation method of acer palmatum

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Embodiment Construction

[0038] In order to make the technical means, creation features, achievement goals and effects of the present invention easy to understand and understand, the present invention will be further described below with reference to specific embodiments.

[0039] refer to figure 1 As shown in the figure, the tissue culture rapid propagation method of Acer chinensis comprises the following steps:

[0040] Step a, prepare sterile active oxygen water

[0041] Take tap water and put it in the first container, start the active oxygen generating device, and input the active oxygen generated by the active oxygen generating device into the first container, and stir the active oxygen in the gap to make the active oxygen fully dissolve in water. Some oxygenated water.

[0042] Step b. Sterilization

[0043] Putting the stem section of the first-generation cultivated Acer chinensis neatly into the second container, and injecting the sterile active oxygen water prepared in the first container...

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Abstract

The invention discloses a tissue culture rapid propagation method of acer palmatum. The method comprises the following steps: preparing sterile ozone water, carrying out sterilization, preparing culture mediums, sterilizing the culture mediums and carrying out propagation and transplanting; the stem of the acer palmatum undergoes the culture of the starting culture medium, the multiplication culture medium and the rooting culture medium in sequence to establish a rapid propagation system of the acer palmatum, so that a completely rooted regenerated plantlet is successfully cultured and the survival rate of the transplanting is up to about 90%. The method is capable of overcoming the defect that the acer palmatum is long in conventional seedling period and low in cutting propagation coefficient, and realizing the industrialized production of the acer palmatum seedlings.

Description

technical field [0001] The invention relates to the technical field of maple propagation, in particular to a tissue culture rapid propagation method of maple hens. Background technique [0002] Acer palmatum, also known as Yafeng, belongs to the genus Acer of the Aceraceae family, and is a valuable ornamental tree species in urban landscapes. It is native to the Yangtze River Basin of China, and is also distributed in Japan and the Korean Peninsula. In my country, it is mainly distributed in the provinces of the Yangtze River Basin, and mostly occurs in the forest margins or sparse forests of mountains and hills below 1200m above sea level. Acer is a small deciduous tree, about 8m high, with dark gray bark, current year branches purple or lavender green; perennial branches light gray, purple or dark purple. Maple leaves are opposite, 7-10cm in diameter, truncate at the base, palmately deeply lobed, lobes 5-9, usually 7 lobes, lobes ovate-oblong or lanceolate, apex acute or...

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李倩中荣立苹李淑顺唐玲
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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