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A method for extracting biologically active substances from eggshell membranes

A bioactive substance and eggshell membrane technology, which is applied in the field of poultry egg processing and utilization, can solve the problems of low extraction enzymatic hydrolysis efficiency and the inability to realize the joint extraction of multiple active substances, so as to simplify the process, reduce production costs and improve efficiency Effect

Inactive Publication Date: 2016-08-03
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The invention provides a method for extracting biologically active substances from eggshell membranes, aiming at solving the problems of low enzymatic hydrolysis efficiency and inability to realize combined extraction of various active substances in existing enzymatic extraction methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Eggshell membrane separation

[0033] Take 2kg of duck eggshells, wash them with tap water, drain the water, and then use a crusher to break the eggshells into small pieces of about 1mm. Add 8 kg of 0.80 mol / L hydrochloric acid solution, stir at room temperature for 60 minutes, and then separate the eggshell membrane by sieving to obtain about 100 g of eggshell membrane.

[0034] 2. Eggshell membrane crushing

[0035] The separated eggshell membrane was washed with clean water to remove the residual acid solution, then drained, and dried at a constant temperature of 55°C for about 8 hours, and then the eggshell membrane was crushed into a fine powder of about 100 mesh with a universal grinder.

[0036] 3. Eggshell membrane ionic liquid treatment

[0037] Add 100g of eggshell membrane powder to 5kg of 1-hydroxyethyl-3-methylimidazolium tetrafluoroborate ionic liquid, stir at 85°C for 4.5h, then sieve to separate the eggshell membrane, and rinse with ethanol to remov...

Embodiment 2

[0048] 1. Eggshell membrane separation

[0049] Take 3kg of egg shells, wash them with tap water, drain the water, and then use a crusher to break the egg shells into small pieces of about 1mm. Add 6 kg of 0.60 mol / L hydrochloric acid solution, stir at room temperature for 40 minutes, and then separate the eggshell membrane by sieving to obtain 120 g of eggshell membrane.

[0050] 2. Eggshell membrane crushing

[0051] The separated eggshell membrane was washed with clean water to remove the residual acid solution, then drained, and dried at a constant temperature of 55°C for about 8 hours, and then the eggshell membrane was crushed into a fine powder of about 100 mesh with a universal grinder.

[0052] 3. Eggshell membrane ionic liquid treatment

[0053] Take 100g of eggshell membrane powder and add 2kg of 1-hydroxyethyl-3-methylimidazolium tetrafluoroborate ionic liquid, stir at 75°C for 6h, then sieve to separate the eggshell membrane, and rinse with ethanol Remove resid...

Embodiment 3

[0064] 1. Eggshell membrane separation

[0065] Take 2kg of duck eggshells, wash them with tap water, drain the water, and then use a crusher to break the eggshells into small pieces of about 1mm. Add 6 kg of 0.70 mol / L hydrochloric acid solution, stir at room temperature for 80 minutes, and then separate the eggshell membrane by sieving to obtain 100 g of eggshell membrane.

[0066] 2. Eggshell membrane crushing

[0067] The separated eggshell membrane was washed with clean water to remove the residual acid solution, then drained, and dried at a constant temperature of 55°C for about 8 hours, and then the eggshell membrane was crushed into a fine powder of about 100 mesh with a universal grinder.

[0068] 3. Eggshell membrane ionic liquid treatment

[0069] Add 3.5kg of 1-hydroxyethyl-3-methylimidazolium tetrafluoroborate to 100g of eggshell membrane powder, stir for 3 hours at 95°C, then sieve to separate the eggshell membrane, and rinse with ethanol to remove the residue ...

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PUM

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Abstract

The invention discloses a method for extracting bioactive substances from an eggshell membrane. The method for extracting the bioactive substances from the eggshell membrane comprises the following steps: separating an eggshell membrane, smashing, carrying out enzymolysis, and extracting the bioactive substances, wherein eggshell membrane powder is treated by adopting ionic liquid before enzymolysis is carried out, and the ionic liquid is 1-hydroxyethyl-3-methylimidazole tetrafluoroborate. The method for extracting the bioactive substances from the eggshell membrane has the advantages that enzymolysis efficiency is improved, combined separation and extraction of keratin, hyaluronic acid and chondroitin sulphate is realized, and bioactive substance extraction also can be simplified, and environmental requirement is met.

Description

technical field [0001] The invention relates to the technical field of poultry egg processing and utilization, in particular to a method for extracting biologically active substances from eggshell membranes. Background technique [0002] Eggshell membrane (commonly known as phoenix coat) is a fibrous film between the eggshell and egg white, accounting for about 4-5% of the weight of the eggshell. It contains about 90% protein, 3% liposome and 2% liposome. carbohydrate. Eggshell membrane proteins include keratin, collagen, sialoglycoprotein, ovalbumin, lysozyme, porphyrin, etc. Eggshell membranes also contain biologically active substances such as N-acetylglucosamine galactose, glucuronic acid, hyaluronic acid, and chondroitin sulfate. These ingredients have important physiological effects on the human body and are valuable raw materials for food, cosmetics, pharmaceuticals, and chemical industries. [0003] my country is the world's largest egg-producing country. In 2013,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08C12P21/06C07K1/30
Inventor 陈有亮胡徐登
Owner ZHEJIANG UNIV
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